HAMAP rule MF_01590
General rule information
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Accession | MF_01590 |
Dates | 22-NOV-2007 (Created)
1-JUN-2023 (Last updated, Version 15) |
Name | tRNA_carboxymethyltr_CmoB |
Scope(s) |
Bacteria Oscillatoriales Pseudomonadota |
Template(s) | P76291 (CMOB_ECOLI); Q8ZNV1 (CMOB_SALTY); [ Recover all ] |
Triggered by |
HAMAP; MF_01590 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | CMOB |
Protein name | RecName: Full=tRNA U34 carboxymethyltransferase; EC=2.5.1.-; |
Gene name | Name=cmoB; |
Comments
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FUNCTION | Catalyzes carboxymethyl transfer from carboxy-S-adenosyl-L- methionine (Cx-SAM) to 5-hydroxyuridine (ho5U) to form 5- carboxymethoxyuridine (cmo5U) at position 34 in tRNAs. |
CATALYTIC ACTIVITY | Reaction=5-hydroxyuridine(34) in tRNA + carboxy-S-adenosyl-L-methionine = 5-carboxymethoxyuridine(34) in tRNA + H(+) + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:52848, Rhea:RHEA-COMP:13381, Rhea:RHEA- COMP:13383, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:134278, ChEBI:CHEBI:136877, ChEBI:CHEBI:136879; |
case <OC:Pseudomonadota> | |
SUBUNIT | Homotetramer. |
end case | |
SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. CmoB family. |
Keywords
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Gene Ontology
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GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups |
GO:0006400; Biological process:tRNA modification |
Cross-references
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Features
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From: CMOB_ECOLI (P76291) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 152 | 154 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
[DE]-P-[TS] | ||||||||
BINDING | 181 | 182 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
[IVLM]-[ED] | ||||||||
BINDING | 91 | 91 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
K | ||||||||
BINDING | 105 | 105 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
W | ||||||||
BINDING | 110 | 110 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
K | ||||||||
BINDING | 130 | 130 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
G | ||||||||
BINDING | 196 | 196 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
M | ||||||||
BINDING | 200 | 200 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
Y | ||||||||
BINDING | 315 | 315 | /ligand="carboxy-S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:134278" |
R |
Additional information
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Size range | 261-363 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |