HAMAP rule MF_01609
General rule information
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| Accession | MF_01609 |
| Dates | 12-APR-2005 (Created)
3-SEP-2024 (Last updated, Version 27) |
| Name | Glu_cys_ligase_2 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | D4GZN2 (GCS2_HALVD); P77213 (GCS2_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01609 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GCS2 |
| case <OC:Archaea> | |
| Protein name | RecName: Full=Glutamate--cysteine ligase; EC=6.3.2.2; AltName: Full=Gamma-glutamylcysteine synthetase; Short=Gamma-GCS; Short=GCS; |
| Gene name | Name=gshA; |
| else | |
| Protein name | RecName: Full=Putative glutamate--cysteine ligase 2; EC=6.3.2.2; AltName: Full=Gamma-glutamylcysteine synthetase 2; Short=Gamma-GCS 2; Short=GCS 2; |
| end case | |
Comments
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| case <OC:Archaea> | |
| FUNCTION | Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC), the main low-molecular-weight thiol compound instead of glutathione in halophilic archaea. |
| else | |
| FUNCTION | ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+); Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2; |
| case <OC:Enterobacterales> | |
| SUBUNIT | Homodimer. |
| end case | |
| SIMILARITY | Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily. |
Keywords
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Gene Ontology
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| case <OCellular component:Archaea> | |
| GO:0004357; Molecular function:glutamate-cysteine ligase activity | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0016879; Molecular function:ligase activity, forming carbon-nitrogen bonds | |
| end case | |
Cross-references
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Features
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Additional information
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| Size range | 320-426 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: <Unknown>; <Peptidase M20> |