HAMAP rule MF_01611
General rule information
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Accession | MF_01611 |
Dates | 8-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 26) |
Name | FO_synth_sub1 |
Scope(s) |
Bacteria Cyanobacteriota Archaea Euryarchaeota |
Template(s) | Q57888 (COFG_METJA); [ Recover all ] |
Triggered by |
HAMAP; MF_01611 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | COFG |
Protein name | RecName: Full=7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase; EC=4.3.1.32; AltName: Full=FO synthase subunit 1; |
Gene name | Name=cofG; |
Comments
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FUNCTION | Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D- ribitylimino)-5,6-dihydrouracil. |
CATALYTIC ACTIVITY | Reaction=5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6- dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8- didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine + NH4(+); Xref=Rhea:RHEA:55204, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:28938, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:59904, ChEBI:CHEBI:85936; EC=4.3.1.32; |
case <FTGroup:1> | |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
end case | |
PATHWAY | Cofactor biosynthesis; coenzyme F0 biosynthesis. |
SUBUNIT | The FO synthase complex consists of two subunits, CofG and CofH. |
SIMILARITY | Belongs to the radical SAM superfamily. CofG family. |
Keywords
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case <FTGroup:1> | |
4Fe-4S | |
Iron | |
Iron-sulfur | |
Metal-binding | |
S-adenosyl-L-methionine | |
end case | |
Lyase |
Gene Ontology
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case <FTGroup:1> | |
GO:0005506; Molecular function:iron ion binding | |
end case | |
GO:0016841; Molecular function:ammonia-lyase activity |
Cross-references
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Features
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From: COFG_METJA (Q57888) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 49 | 49 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
BINDING | 53 | 53 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
BINDING | 56 | 56 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 |
Additional information
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Size range | 313-380 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: MF_01612 (cofH) |
Comments | Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts. |