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Annotation rule MF_01611
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General rule information [?]

Accession MF_01611
Dates 8-APR-2005 (Created)
10-OCT-2014 (Last updated, Version 18)
Name FO_synth_sub1
Scope Bacteria; Cyanobacteria
Archaea; Euryarchaeota
Template Q57888 (COFG_METJA)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
COFG
Protein name
RecName: Full=FO synthase subunit 1;
EC=2.5.1.77;
AltName: Full=7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1;
Gene name
cofG

Comments [?]

Function Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO).
Catalytic activity 5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H(2)O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH(3).
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
end case
Pathway Cofactor biosynthesis; coenzyme F0 biosynthesis.
Subunit Consists of two subunits, CofG and CofH.
Similarity Belongs to the radical SAM superfamily. CofG family.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0005506; Molecular function: iron ion binding.
end case
GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.
GO:0009108; Biological process: coenzyme biosynthetic process.

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
TIGRFAMs TIGR03550; F420_cofG; 1;

Features [?]

From: COFG_METJA (Q57888)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     49     49       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1
METAL     53     53       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1
METAL     56     56       Iron-sulfur (4Fe-4S-S-AdoMet)     C   1

Additional information [?]

Size range 313-380 amino acids
Related rules None
Fusion Nter: None; Cter: MF_01612 (cofH)
Comments Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts.