HAMAP rule MF_01612
General rule information
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| Accession | MF_01612 |
| Dates | 11-APR-2005 (Created)
1-JUN-2023 (Last updated, Version 26) |
| Name | FO_synth_sub2 |
| Scope(s) |
Bacteria Cyanobacteriota Archaea Euryarchaeota |
| Template(s) | Q58826 (COFH_METJA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01612 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | COFH |
| Protein name | RecName: Full=5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase; EC=2.5.1.147; AltName: Full=FO synthase subunit 2; |
| Gene name | Name=cofH; |
Comments
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| FUNCTION | Catalyzes the radical-mediated synthesis of 5-amino-5-(4- hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D- ribitylamino)uracil and L-tyrosine. |
| CATALYTIC ACTIVITY | Reaction=5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L- methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4- hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L- methionine; Xref=Rhea:RHEA:55200, ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:17319, ChEBI:CHEBI:57844, ChEBI:CHEBI:58315, ChEBI:CHEBI:59789, ChEBI:CHEBI:77846, ChEBI:CHEBI:85936; EC=2.5.1.147; |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| end case | |
| PATHWAY | Cofactor biosynthesis; coenzyme F0 biosynthesis. |
| SUBUNIT | The FO synthase complex consists of two subunits, CofG and CofH. |
| SIMILARITY | Belongs to the radical SAM superfamily. CofH family. |
Keywords
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| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| S-adenosyl-L-methionine | |
| end case | |
| Transferase | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0005506; Molecular function:iron ion binding | |
| end case | |
| GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups | |
Cross-references
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| Pfam | PF04055; Radical_SAM; 1; |
| PIRSF | PIRSF004762; CHP00423; 1; |
| NCBIfam | TIGR00423; TIGR00423; 1; |
Features
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| From: COFH_METJA (Q58826) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 59 | 59 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 63 | 63 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 66 | 66 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
Additional information
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| Size range | 338-406 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_01611 (cofG) Cter: None |
| Comments | Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts. |