HAMAP

Accession	MF_01612
Dates	11-APR-2005 (Created) 7-OCT-2011 (Last updated, Version 16)

Data class

Protein

Predictors

HAMAP; MF_01612; [distribution of match scores in UniProtKB];[seed alignment for MF_01612]

Names

FO_synth_sub2

Identifier

COFH

Protein name

RecName: Full=FO synthase subunit 2; EC=2.5.1.77; AltName: Full=7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2;

Gene name

cofH

FUNCTION: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) (By similarity).

CATALYTIC ACTIVITY: 5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H(2)O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH(3).

COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).

PATHWAY: Cofactor biosynthesis; coenzyme F0 biosynthesis.

SUBUNIT: Consists of two subunits, CofG and CofH (By similarity).

SIMILARITY: Belongs to the radical SAM superfamily. CofH family.

Pfam	PF04055; Radical_SAM; 1;
PIRSF	PIRSF004762; CHP00423; 1;
TIGRFAMs	TIGR00423; TIGR00423; 1;

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine.

Transferase.

GO:0005506; Molecular function: iron ion binding.

GO:0016765; Molecular function: transferase activity, transferring alkyl or aryl (other than methyl) groups.
GO:0009108; Biological process: coenzyme biosynthetic process.

From: COFH_METJA (Q58826)
Key		From		To		Description		Condition		FTGroup
METAL		59		59		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		1
METAL		63		63		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		1
METAL		66		66		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		1

Size range:	338-406 amino acids
Related UniRules:	None
Template:	Q58826 (COFH_METJA)
Scope:	Bacteria; Cyanobacteria Archaea; Euryarchaeota
Fusion:	Nter: MF_01611 (cofG); Cter: None
Duplicate:	in METAC, METMA, METMP
Plasmid encoded:	None
Comments:	Euryarchaeota, cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts.