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HAMAP rule MF_01615

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General rule information [?]

Accession MF_01615
Dates 13-JUN-2005 (Created)
1-JUN-2023 (Last updated, Version 26)
Name PdxT
Scope(s) Bacteria
Archaea
Template(s) P37528 (PDXT_BACSU); Q9WYU3 (PDXT_THEMA); [ Recover all ]
Triggered by HAMAP; MF_01615 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PDXT
Protein name RecName: Full=Pyridoxal 5'-phosphate synthase subunit PdxT;
                 EC=4.3.3.6;
AltName: Full=Pyridoxal 5'-phosphate synthase glutaminase subunit;
                 EC=3.5.1.2;
AltName: Full=Pdx2;
Gene name Name=pdxT;

Comments [?]

FUNCTIONCatalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.
CATALYTIC ACTIVITY Reaction=aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'- phosphate; Xref=Rhea:RHEA:31507, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:43474, ChEBI:CHEBI:58273, ChEBI:CHEBI:58359, ChEBI:CHEBI:59776, ChEBI:CHEBI:597326; EC=4.3.3.6;
CATALYTIC ACTIVITY Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
PATHWAYCofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.
SUBUNITIn the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.
SIMILARITYBelongs to the glutaminase PdxT/SNO family.

Keywords [?]


Gene Ontology [?]

GO:0004359; Molecular function:glutaminase activity
GO:0036381; Molecular function:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
GO:0006543; Biological process:glutamine catabolic process
GO:0042823; Biological process:pyridoxal phosphate biosynthetic process

Cross-references [?]

Pfam PF01174; SNO; 1;
PRINTS PR00096; GATASE; 1;
PROSITE PS01236; PDXT_SNO_1; 1;
PROSITE PS51130; PDXT_SNO_2; 1;
NCBIfam TIGR03800; PLP_synth_Pdx2; 1;

Features [?]

From: PDXT_BACSU (P37528)
Key From To Description Tag Condition FTGroup
BINDING 47 49 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
G-x-S
BINDING 134 135 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
I-R
ACT_SITE 79 79 /note="Nucleophile" C
ACT_SITE 170 170 /note="Charge relay system" H
ACT_SITE 172 172 /note="Charge relay system" E
BINDING 106 106 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
R

Additional information [?]

Size range 173-236 amino acids
Related rules None
Fusion Nter: None Cter: None



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