HAMAP

Accession	MF_01617
Dates	11-NOV-2005 (Created) 25-NOV-2009 (Last updated, Version 18)

Data class

Protein

Predictors

HAMAP; MF_01617; [distribution of match scores in UniProtKB];[seed alignment for MF_01617]

Names

FadJ

Identifier

FADJ

Protein name

RecName: Full=Fatty acid oxidation complex subunit alpha; RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; EC=4.2.1.17; EC=5.1.2.3; RecName: Full=3-hydroxyacyl-CoA dehydrogenase; EC=1.1.1.35;

Gene name

fadJ

FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By similarity).

CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.

CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.

CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.

PATHWAY: Lipid metabolism; fatty acid beta-oxidation.

SUBUNIT: Heterotetramer of two alpha chains (fadJ) and two beta chains (fadI) (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

PROSITE	PS00067; 3HCDH; 1; PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam	PF00725; 3HCDH; 2; PF02737; 3HCDH_N; 1; PF00378; ECH; 1;
TIGRFAMs	TIGR02440; FadJ; 1;

Cytoplasm, Fatty acid metabolism, Isomerase, Lipid degradation, Lipid metabolism, Lyase, Multifunctional enzyme, NAD, Oxidoreductase.

GO:0003857; Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity.
GO:0004300; Molecular function: enoyl-CoA hydratase activity.
GO:0008692; Molecular function: 3-hydroxybutyryl-CoA epimerase activity.
GO:0006631; Biological process: fatty acid metabolic process.
GO:0016042; Biological process: lipid catabolic process.
GO:0005737; Cellular component: cytoplasm.

From: FADJ_ECOLI (P77399)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		190		Enoyl-CoA hydratase (By similarity)
REGION		306		Cter		3-hydroxyacyl-CoA dehydrogenase (By similarity)
SITE		118		118		Important for catalytic activity (By similarity)		E
SITE		140		140		Important for catalytic activity (By similarity)		E

Size range:	699-780 amino acids
Related UniRules:	None
Template:	P77399 (FADJ_ECOLI)
Scope:	Bacteria; Gammaproteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None