HAMAP rule MF_01622
General rule information
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| Accession | MF_01622 |
| Dates | 21-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 24) |
| Name | tRNA_sel_U_synth |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P33667 (SELU_ECOLI); Q8ZR88 (SELU_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01622 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SELU |
| Protein name | RecName: Full=tRNA 2-selenouridine synthase; EC=2.9.1.3; |
| Gene name | Name=selU; |
Comments
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| FUNCTION | Involved in the post-transcriptional modification of the uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Catalyzes the conversion of 2-thiouridine (S2U-RNA) to 2- selenouridine (Se2U-RNA). Acts in a two-step process involving geranylation of 2-thiouridine (S2U) to S-geranyl-2-thiouridine (geS2U) and subsequent selenation of the latter derivative to 2-selenouridine (Se2U) in the tRNA chain. |
| CATALYTIC ACTIVITY | Reaction=(2E)-geranyl diphosphate + 5-methylaminomethyl-2- thiouridine(34) in tRNA + H(+) + H2O + selenophosphate = (2E)- thiogeraniol + 5-methylaminomethyl-2-selenouridine(34) in tRNA + diphosphate + phosphate; Xref=Rhea:RHEA:42716, Rhea:RHEA-COMP:10195, Rhea:RHEA-COMP:10196, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16144, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474, ChEBI:CHEBI:58057, ChEBI:CHEBI:74455, ChEBI:CHEBI:82743, ChEBI:CHEBI:143703; EC=2.9.1.3; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42717; |
| CATALYTIC ACTIVITY | Reaction=(2E)-geranyl diphosphate + 5-methylaminomethyl-2- thiouridine(34) in tRNA = 5-methylaminomethyl-S-(2E)-geranyl- thiouridine(34) in tRNA + diphosphate; Xref=Rhea:RHEA:14085, Rhea:RHEA-COMP:10195, Rhea:RHEA-COMP:14654, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057, ChEBI:CHEBI:74455, ChEBI:CHEBI:140632; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14086; |
| CATALYTIC ACTIVITY | Reaction=5-methylaminomethyl-S-(2E)-geranyl-thiouridine(34) in tRNA + H(+) + selenophosphate = (2E)-thiogeraniol + 5-methylaminomethyl-2- (Se-phospho)selenouridine(34) in tRNA; Xref=Rhea:RHEA:60172, Rhea:RHEA-COMP:14654, Rhea:RHEA-COMP:15523, ChEBI:CHEBI:15378, ChEBI:CHEBI:16144, ChEBI:CHEBI:140632, ChEBI:CHEBI:143702, ChEBI:CHEBI:143703; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60173; |
| CATALYTIC ACTIVITY | Reaction=5-methylaminomethyl-2-(Se-phospho)selenouridine(34) in tRNA + H2O = 5-methylaminomethyl-2-selenouridine(34) in tRNA + phosphate; Xref=Rhea:RHEA:60176, Rhea:RHEA-COMP:10196, Rhea:RHEA-COMP:15523, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:82743, ChEBI:CHEBI:143702; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60177; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the SelU family. |
Keywords
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Gene Ontology
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| GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups |
| GO:0002098; Biological process:tRNA wobble uridine modification |
Cross-references
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Features
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| From: SELU_ECOLI (P33667) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 97 | 97 | /note="S-selanylcysteine intermediate" | C | ||||||||
Additional information
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| Size range | 350-410 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |