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HAMAP annotation rule: MF_01623
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General rule information

Accession MF_01623
Dates 23-NOV-2005 (Created)
25-NOV-2009 (Last updated, Version 11)
Data class Protein
Predictors HAMAP; MF_01623; [distribution of match scores in UniProtKB];[seed alignment for MF_01623]
Names MepA


Propagated annotation

Identifier, protein and gene names
Identifier MEPA
Protein name
RecName: Full=Penicillin-insensitive murein endopeptidase;
EC=3.4.24.-;
AltName: Full=D-alanyl-D-alanine-endopeptidase;
Short=DD-endopeptidase;
Flags: Precursor;
Gene name mepA
Comments
FUNCTION: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein (By similarity).
CATALYTIC ACTIVITY: Splits the D-alanyl-gamma-meso-2,6-diamino-pimelyl peptide bond connecting neighboring peptidoglycan strands.
COFACTOR: Binds 2 zinc ions per subunit. Zinc ion 1 is bound in the active site. Zinc ion 2 is bound at the dimer interface by residues from both subunits (By similarity).
SUBUNIT: Dimer (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
SIMILARITY: Belongs to the peptidase M74 family.
Cross-references
Pfam PF03411; Peptidase_M74; 1;
PIRSF PIRSF018455; MepA; 1;
General Signal; -; 1; trigger=Yes;
Keywords

case <FT:7> or <FT:8> or <FT:9>
Disulfide bond.
end case

Hydrolase, Metal-binding, Metalloprotease, Periplasm, Protease, Signal, Zinc.
Gene Ontology
GO:0004222; Molecular function: metalloendopeptidase activity.
GO:0000270; Biological process: peptidoglycan metabolic process.
GO:0042597; Cellular component: periplasmic space.
Features
From: MEPA_ECOLI (P0C0T5)
Key     From     To       Description   Condition   FTGroup
METAL     110     110       Zinc 1 (By similarity)   H  
METAL     113     113       Zinc 1 (By similarity)   H  
METAL     120     120       Zinc 1 (By similarity)   D  
METAL     147     147       Zinc 2 (By similarity)   D  
METAL     150     150       Zinc 2 (By similarity)   H  
METAL     211     211       Zinc 1 (By similarity)   H  
DISULFID     44     265       By similarity   C-x*-C  
DISULFID     187     235       By similarity   C-x*-C  
DISULFID     216     223       By similarity   C-x*-C  


Additional information

Size range: 274-281 amino acids
Related UniRules: None
Template: P0C0T5 (MEPA_ECOLI)
Scope: Bacteria; Enterobacteriaceae
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None

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UniProtKB rule member sequences