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HAMAP rule MF_01627

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General rule information [?]

Accession MF_01627
Dates 13-DEC-2005 (Created)
1-JUN-2023 (Last updated, Version 25)
Name Pur_nucleosid_phosp
Scope(s) Bacteria
Bacillota
Pseudomonadota
Template(s) P0ABP8 (DEOD_ECOLI); Q8ZJV7 (DEOD_SALTY); [ Recover all ]
Triggered by HAMAP; MF_01627 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DEOD
Protein name RecName: Full=Purine nucleoside phosphorylase DeoD-type;
                 Short=PNP;
                 EC=2.4.2.1;
Gene name Name=deoD;

Comments [?]

FUNCTIONCatalyzes the reversible phosphorolytic breakdown of the N- glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1- phosphate.
CATALYTIC ACTIVITY Reaction=a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate; Xref=Rhea:RHEA:19805, ChEBI:CHEBI:26386, ChEBI:CHEBI:43474, ChEBI:CHEBI:57720, ChEBI:CHEBI:142355; EC=2.4.2.1;
CATALYTIC ACTIVITY Reaction=a purine 2'-deoxy-D-ribonucleoside + phosphate = 2-deoxy- alpha-D-ribose 1-phosphate + a purine nucleobase; Xref=Rhea:RHEA:36431, ChEBI:CHEBI:26386, ChEBI:CHEBI:43474, ChEBI:CHEBI:57259, ChEBI:CHEBI:142361; EC=2.4.2.1;
SUBUNITHomohexamer; trimer of homodimers.
SIMILARITYBelongs to the PNP/UDP phosphorylase family.

Keywords [?]

case <FTTag:acetylation>
Acetylation
end case
Glycosyltransferase
Transferase

Gene Ontology [?]

GO:0004731; Molecular function:purine-nucleoside phosphorylase activity
GO:0042278; Biological process:purine nucleoside metabolic process

Cross-references [?]

PROSITE PS01232; PNP_UDP_1; 1;
Pfam PF01048; PNP_UDP_1; 1;
NCBIfam TIGR00107; deoD; 1;

Features [?]

From: DEOD_ECOLI (P0ABP8)
Key From To Description Tag Condition FTGroup
ACT_SITE 205 205 /note="Proton donor" D
SITE 218 218 /note="Important for catalytic activity" R
BINDING 88 91 /ligand="phosphate"
/ligand_id="ChEBI:CHEBI:43474"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
R-[IV]-G-[TS]
BINDING 180 182 /ligand="a purine D-ribonucleoside"
/ligand_id="ChEBI:CHEBI:142355"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
[ED]-M-E
BINDING 204 205 /ligand="a purine D-ribonucleoside"
/ligand_id="ChEBI:CHEBI:142355"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
[ST]-[DN]
BINDING 5 5 /ligand="a purine D-ribonucleoside"
/ligand_id="ChEBI:CHEBI:142355"
/ligand_note="ligand shared between dimeric partners"
H
BINDING 21 21 /ligand="phosphate"
/ligand_id="ChEBI:CHEBI:43474"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
G
BINDING 25 25 /ligand="phosphate"
/ligand_id="ChEBI:CHEBI:43474"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
R
BINDING 44 44 /ligand="phosphate"
/ligand_id="ChEBI:CHEBI:43474"
/ligand_note="ligand shared between dimeric partners"
R
case <OC:Escherichia> or <OC:Shigella>
MOD_RES 27 27 /note="N6-acetyllysine" acetylation K
end case

Additional information [?]

Size range 220-270 amino acids
Related rules None
Fusion Nter: None Cter: None



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