HAMAP

Accession	MF_01631
Dates	4-APR-2006 (Created) 19-AUG-2011 (Last updated, Version 27)

Data class

Protein

Predictors

HAMAP; MF_01631; [distribution of match scores in UniProtKB];[seed alignment for MF_01631]

Names

GlmU

Identifier

GLMU

Protein name

RecName: Full=Bifunctional protein GlmU; RecName: Full=UDP-N-acetylglucosamine pyrophosphorylase; EC=2.7.7.23; AltName: Full=N-acetylglucosamine-1-phosphate uridyltransferase; RecName: Full=Glucosamine-1-phosphate N-acetyltransferase; EC=2.3.1.157;

Gene name

glmU

FUNCTION: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc (By similarity).

CATALYTIC ACTIVITY: Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.

CATALYTIC ACTIVITY: UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.

PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.

PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.

SIMILARITY: In the C-terminal section; belongs to the transferase hexapeptide repeat family.

PROSITE	PS00101; HEXAPEP_TRANSFERASES; 1;
Pfam	PF00132; Hexapep; 7; PF00483; NTP_transferase; 1; PF01128; IspD; 1; PF02348; CTP_transf_3; 1;
PRINTS	PR01590; HTHFIS; 1; PR01415; ANKYRIN; 1;
TIGRFAMs	TIGR01173; GlmU; 1;

Cytoplasm, Acyltransferase, Cell shape, Cell wall biogenesis/degradation, Magnesium, Metal-binding, Multifunctional enzyme, Nucleotidyltransferase, Peptidoglycan synthesis, Repeat, Transferase.

GO:0000287; Molecular function: magnesium ion binding.
GO:0003977; Molecular function: UDP-N-acetylglucosamine diphosphorylase activity.
GO:0019134; Molecular function: glucosamine-1-phosphate N-acetyltransferase activity.
GO:0000902; Biological process: cell morphogenesis.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: GLMU_ECOLI (P0ACC7)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		229		Pyrophosphorylase (By similarity)
REGION (Optional)		11		14		Substrate binding (By similarity)		L-A-A-G
REGION (Optional)		81		82		Substrate binding (By similarity)		G-T
REGION		230		250		Linker (By similarity)
REGION		251		Cter		N-acetyltransferase (By similarity)
METAL		105		105		Magnesium (By similarity)		D
METAL		227		227		Magnesium (By similarity)		N
ACT_SITE		363		363		Proton acceptor (By similarity)		H
BINDING (Optional)		76		76		Substrate (By similarity)		Q
BINDING (Optional)		140		140		Substrate; via amide nitrogen (By similarity)		G
BINDING (Optional)		154		154		Substrate (By similarity)		E
BINDING (Optional)		169		169		Substrate (By similarity)		N
BINDING (Optional)		387		387		Acetyl-CoA (By similarity)		Y
BINDING (Optional)		405		405		Acetyl-CoA (By similarity)		S
BINDING (Optional)		423		423		Acetyl-CoA; via amide nitrogen (By similarity)		[ACGST]
BINDING (Optional)		440		440		Acetyl-CoA (By similarity)		R

Size range:	339-500 amino acids
Related UniRules:	None
Template:	P0ACC7 (GLMU_ECOLI); Q97R46 (GLMU_STRPN): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: <Unknown>
Duplicate:	None
Plasmid encoded:	None
Comments:	SYNJA and SYNJB are fusioned in their C-terminal parts to an unknown domain.