HAMAP

Accession	MF_01643
Dates	24-JAN-2008 (Created) 18-AUG-2010 (Last updated, Version 12)

Data class

Protein

Predictors

HAMAP; MF_01643; [distribution of match scores in UniProtKB];[seed alignment for MF_01643]

Names

PurT

Identifier

PURT

Protein name

RecName: Full=Phosphoribosylglycinamide formyltransferase 2; Short=GART 2; EC=2.1.2.-; AltName: Full=5'-phosphoribosylglycinamide transformylase 2; AltName: Full=Formate-dependent GAR transformylase; AltName: Full=GAR transformylase 2;

Gene name

purT

FUNCTION: Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP (By similarity).

CATALYTIC ACTIVITY: Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.

PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the purK/purT family.

SIMILARITY: Contains 1 ATP-grasp domain.

PROSITE	PS50975; ATP_GRASP; 1;
Pfam	PF02222; ATP-grasp; 1;
TIGRFAMs	TIGR01142; purT; 1;

Acetylation.

ATP-binding, Metal-binding, Magnesium, Nucleotide-binding, Purine biosynthesis, Transferase.

GO:0000166; Molecular function: nucleotide binding.
GO:0005524; Molecular function: ATP binding.
GO:0043815; Molecular function: phosphoribosylglycinamide formyltransferase 2 activity.

From: PURT_ECOLI (P33221)
Key		From		To		Description		Condition		FTGroup
DOMAIN		119		308		ATP-grasp

Key		From		To		Description		Condition		FTGroup
NP_BIND		160		165		ATP (By similarity)		S-S-G-x-G-Q
NP_BIND		195		198		ATP (By similarity)		E-x-x-[VIAL]
REGION		22		23		5'-phosphoribosylglycinamide binding (By similarity)		E-L
REGION		362		363		5'-phosphoribosylglycinamide binding (By similarity)		R-R
METAL		267		267		Magnesium (By similarity)		E
METAL		279		279		Magnesium (By similarity)		[ED]
BINDING		82		82		5'-phosphoribosylglycinamide (By similarity)		E
BINDING		114		114		ATP (By similarity)		R
BINDING		155		155		ATP (By similarity)		K
BINDING		203		203		ATP (By similarity)		E
BINDING		267		267		ATP (By similarity)		E
BINDING		279		279		ATP (By similarity)		[ED]
BINDING		286		286		5'-phosphoribosylglycinamide (By similarity)		D
BINDING		355		355		5'-phosphoribosylglycinamide (By similarity)		K

Key		From		To		Description		Condition		FTGroup
MOD_RES		179		179		N6-acetyllysine (By similarity)		K

Size range:	379-439 amino acids
Related UniRules:	None
Template:	P33221 (PURT_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None