Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
HAMAP rule MF_01654
General rule information
[?]
| Accession | MF_01654 |
| Dates | 30-APR-2008 (Created)
12-MAR-2024 (Last updated, Version 22) |
| Name | MhpC |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P77044 (MHPC_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01654 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | MHPC |
| Protein name | RecName: Full=2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase; EC=3.7.1.14; AltName: Full=2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; AltName: Full=2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; AltName: Full=2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; |
| Gene name | Name=mhpC; |
Comments
[?]
| FUNCTION | Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid. |
| CATALYTIC ACTIVITY | Reaction=(2Z,4E)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O = (2Z)-2- hydroxypenta-2,4-dienoate + H(+) + succinate; Xref=Rhea:RHEA:34187, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031, ChEBI:CHEBI:66887, ChEBI:CHEBI:67152; EC=3.7.1.14; |
| CATALYTIC ACTIVITY | Reaction=(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-trienedioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate + H(+); Xref=Rhea:RHEA:34191, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29806, ChEBI:CHEBI:66888, ChEBI:CHEBI:67152; EC=3.7.1.14; |
| PATHWAY | Aromatic compound metabolism; 3-phenylpropanoate degradation. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the AB hydrolase superfamily. MhpC family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0018771; Molecular function:2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity |
| GO:0019380; Biological process:3-phenylpropionate catabolic process |
Cross-references
[?]
Features
[?]
| From: MHPC_ECOLI (P77044) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 267 | 267 | /note="Proton acceptor" | H | ||||||||
| SITE | 114 | 114 | /note="Transition state stabilizer" | S | ||||||||
| SITE | 192 | 192 | /note="Catalytic role in ketonization of the dienol substrate (substrate destabilization)" | R | ||||||||
Additional information
[?]
| Size range | 285-296 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | BURCE and COMTE are tagged as atypical due to their sequence similarity to BphD. |