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HAMAP annotation rule: MF_01654
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General rule information

Accession MF_01654
Dates 30-APR-2008 (Created)
3-NOV-2011 (Last updated, Version 14)
Data class Protein
Predictors HAMAP; MF_01654; [distribution of match scores in UniProtKB];[seed alignment for MF_01654]
Names MhpC


Propagated annotation

Identifier, protein and gene names
Identifier MHPC
Protein name
RecName: Full=2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase;
EC=3.7.1.14;
AltName: Full=2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase;
AltName: Full=2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase;
AltName: Full=2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase;
Gene name mhpC
Comments
FUNCTION: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid (By similarity).
CATALYTIC ACTIVITY: (2E,4Z)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H(2)O = (2E)-2-hydroxypenta-2,4-dienoate + succinate.
CATALYTIC ACTIVITY: (2E,4Z,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H(2)O = (2E)-2-hydroxypenta-2,4-dienoate + fumarate.
PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the mhpC alpha/beta hydrolase family.
Cross-references
Pfam PF00561; Abhydrolase_1; 1;
PRINTS PR00111; ABHYDROLASE; 1;
PR00412; EPOXHYDRLASE; 1;
Keywords
Aromatic hydrocarbons catabolism, Hydrolase.
Gene Ontology
GO:0018771; Molecular function: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity.
GO:0019439; Biological process: aromatic compound catabolic process.
Features
From: MHPC_ECOLI (P77044)
Key     From     To       Description   Condition   FTGroup
ACT_SITE     267     267       Proton acceptor (By similarity)   H  
SITE     114     114       Transition state stabilizer (By similarity)   S  
SITE     192     192       Catalytic role in ketonization of the dienol substrate (substrate destabilization) (By similarity)   R  


Additional information

Size range: 285-296 amino acids
Related UniRules: None
Template: P77044 (MHPC_ECOLI)
Scope: Bacteria; Proteobacteria
Fusion: Nter: None; Cter: None
Duplicate: in DECAR, PSEPU
Plasmid encoded: None
Comments: BURCE and COMTE are tagged as atypical due to their sequence similarity to BphD.

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UniProtKB rule member sequences