HAMAP

Accession	MF_01656
Dates	30-APR-2008 (Created) 3-OCT-2011 (Last updated, Version 17)

Data class

Protein

Predictors

HAMAP; MF_01656; [distribution of match scores in UniProtKB];[seed alignment for MF_01656]

Names

HOA

Identifier

HOA

Protein name

RecName: Full=4-hydroxy-2-oxovalerate aldolase; Short=HOA; EC=4.1.3.39; AltName: Full=4-hydroxy-2-keto-pentanoic acid aldolase; AltName: Full=4-hydroxy-2-oxopentanoate aldolase;

Gene name

mhpE

FUNCTION: Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds (By similarity).

CATALYTIC ACTIVITY: 4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.

PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.

SUBUNIT: Interacts with mhpF (By similarity).

SIMILARITY: Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

PROSITE	PS50991; PYR_CT; 1;
Pfam	PF07836; DmpG_comm; 1; PF00682; HMGL-like; 1;
TIGRFAMs	TIGR03217; 4OH_2_O_val_ald; 1;

Aromatic hydrocarbons catabolism, Lyase, Manganese, Metal-binding.

GO:0008701; Molecular function: 4-hydroxy-2-oxovalerate aldolase activity.
GO:0030145; Molecular function: manganese ion binding.
GO:0019439; Biological process: aromatic compound catabolic process.

From: HOA_PSEUF (P51016)
Key		From		To		Description		Condition		FTGroup
REGION		17		18		Substrate binding (By similarity)		R-D
ACT_SITE		21		21		Proton acceptor (Potential)		H
METAL		18		18		Manganese (By similarity)		D
METAL		200		200		Manganese; via tele nitrogen (By similarity)		H
METAL		202		202		Manganese; via tele nitrogen (By similarity)		H
BINDING		171		171		Substrate (By similarity)		S
BINDING		200		200		Substrate (By similarity)		H
BINDING		291		291		Substrate (By similarity)		Y
SITE		17		17		Transition state stabilizer (By similarity)		R

Size range:	333-377 amino acids
Related UniRules:	None
Template:	P51020 (HOA_ECOLI); P51015 (HOA4_BURXL); Q9KWS0 (HOA_PSESP); P51014 (HOA_PSES1); Q51983 (HOA2_PSEP1); P51016 (HOA_PSEUF); P51018 (HOA1_PSEP1); P51019 (HOA2_PSEPU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in AZOSB, AZOVD, BURCJ, BURP8, BURS3, BURVG, BURXL, COMTE, DECAR, FRASN, METPP, MYCA9, MYCMM, MYCS2, MYCSJ, MYCSK, MYCSS, MYCUA, MYCVP, NOCFA, NOCSJ, NOVAD, PSEP1, PSEPS, PSEPW, CUPNH, RALME, RHOE4, RHOOB, RHOSR, SALAI, SPHWW
Plasmid encoded:	in GEOSE, BURVG, MYCSK, MYCSS, NOVAD, POLNA, POLSJ, PSEPU, PSEUF, RALME, RALSO, RHOOB, RHOSR, SPHAR, SPHWW, STRCO, THET8
Comments:	This protein is present in multiple copies in some organisms, each one being located in a cluster involved in the degragation of a different aromatic compound. The E.coli protein showed no observable dependence on divalent metal ions (and no loss of enzyme activity by treatment with EDTA) unlike the purified Pseudomonas sp. strain CF600 and Burkholderia xenovorans aldolases.