HAMAP

Accession	MF_01659
Dates	23-MAY-2008 (Created) 19-AUG-2011 (Last updated, Version 11)

Data class

Protein

Predictors

HAMAP; MF_01659; [distribution of match scores in UniProtKB];[seed alignment for MF_01659]

Names

MenD

Identifier

MEND

Protein name

RecName: Full=2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; Short=SEPHCHC synthase; EC=2.2.1.9; AltName: Full=Menaquinone biosynthesis protein MenD;

Gene name

menD

FUNCTION: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) (By similarity).

CATALYTIC ACTIVITY: Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO(2).

COFACTOR: Magnesium or manganese (By similarity).

COFACTOR: Binds 1 thiamine pyrophosphate per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the TPP enzyme family. MenD subfamily.

Pfam	PF02776; TPP_enzyme_N; 1;
TIGRFAMs	TIGR00173; MenD; 1;

Magnesium, Manganese, Menaquinone biosynthesis, Metal-binding, Thiamine pyrophosphate, Transferase.

GO:0000287; Molecular function: magnesium ion binding.
GO:0030145; Molecular function: manganese ion binding.
GO:0030976; Molecular function: thiamine pyrophosphate binding.
GO:0009234; Biological process: menaquinone biosynthetic process.

Size range:	508-619 amino acids
Related UniRules:	None
Template:	P17109 (MEND_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None