HAMAP rule MF_01682
General rule information
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Accession | MF_01682 |
Dates | 4-NOV-2008 (Created)
19-NOV-2022 (Last updated, Version 20) |
Name | Salvage_MtnD |
Scope(s) |
Bacteria |
Template(s) | Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU); [ Recover all ] |
Triggered by |
HAMAP; MF_01682 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | MTND |
Protein name | RecName: Full=Acireductone dioxygenase; AltName: Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Short=DHK-MTPene dioxygenase; AltName: Full=Acireductone dioxygenase (Fe(2+)-requiring); Short=ARD'; Short=Fe-ARD; EC=1.13.11.54; AltName: Full=Acireductone dioxygenase (Ni(2+)-requiring); Short=ARD; Short=Ni-ARD; EC=1.13.11.53; |
Gene name | Name=mtnD; |
Comments
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FUNCTION | Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. |
CATALYTIC ACTIVITY | Reaction=1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3- (methylsulfanyl)propanoate + CO + formate + 2 H(+); Xref=Rhea:RHEA:14161, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:17245, ChEBI:CHEBI:49016, ChEBI:CHEBI:49252; EC=1.13.11.53; |
CATALYTIC ACTIVITY | Reaction=1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4- methylsulfanyl-2-oxobutanoate + formate + 2 H(+); Xref=Rhea:RHEA:24504, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:16723, ChEBI:CHEBI:49252; EC=1.13.11.54; |
COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) cation per monomer.; |
COFACTOR | Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Note=Binds 1 nickel ion per monomer.; |
PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. |
SUBUNIT | Monomer. |
SIMILARITY | Belongs to the acireductone dioxygenase (ARD) family. |
Keywords
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Amino-acid biosynthesis |
Dioxygenase |
Iron |
Metal-binding |
Methionine biosynthesis |
Nickel |
Oxidoreductase |
Gene Ontology
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GO:0005506; Molecular function:iron ion binding |
GO:0010308; Molecular function:acireductone dioxygenase (Ni2+-requiring) activity |
GO:0010309; Molecular function:acireductone dioxygenase [iron(II)-requiring] activity |
GO:0016151; Molecular function:nickel cation binding |
GO:0019284; Biological process:L-methionine salvage from S-adenosylmethionine |
Cross-references
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Pfam | PF03079; ARD; 1; |
Features
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From: MTND_KLEOX (Q9ZFE7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 97 | 97 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" |
H | ||||||||
BINDING | 97 | 97 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
BINDING | 99 | 99 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" |
H | ||||||||
BINDING | 99 | 99 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
BINDING | 103 | 103 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" |
E | ||||||||
BINDING | 103 | 103 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
E | ||||||||
BINDING | 141 | 141 | /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033" |
H | ||||||||
BINDING | 141 | 141 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
SITE | 96 | 96 | /note="May play a role in metal incorporation in vivo" | E | ||||||||
SITE | 102 | 102 | /note="May play a role in transmitting local conformational changes" | D | ||||||||
SITE | 105 | 105 | /note="Important to generate the dianion" | R |
Additional information
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Size range | 165-206 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |