Annotation rule MF_01682
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General rule information [?]

Accession MF_01682
Dates 4-NOV-2008 (Created)
21-MAR-2014 (Last updated, Version 12)
Name Salvage_MtnD
Scope Bacteria

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MTND
Protein name
RecName: Full=Acireductone dioxygenase;
AltName: Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase;
Short=DHK-MTPene dioxygenase;
AltName: Full=Acireductone dioxygenase (Fe(2+)-requiring);
Short=ARD';
Short=Fe-ARD;
EC=1.13.11.54;
AltName: Full=Acireductone dioxygenase (Ni(2+)-requiring);
Short=ARD;
Short=Ni-ARD;
EC=1.13.11.53;
Gene name
mtnD

Comments [?]

Function Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity).
Catalytic activity 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 3-(methylthio)propanoate + formate + CO.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 4-(methylthio)-2-oxobutanoate + formate.
Cofactor Binds 1 iron ion per monomer (By similarity).
Binds 1 nickel ion per monomer (By similarity).
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate; step 5/6.
Subunit Monomer (By similarity).
Similarity Belongs to the acireductone dioxygenase (ARD) family.

Keywords [?]


Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0016151; Molecular function: nickel cation binding.
GO:0019284; Biological process: L-methionine biosynthetic process from S-adenosylmethionine.

Cross-references [?]

Pfam PF03079; ARD; 1;

Features [?]

From: MTND_KLEOX (Q9ZFE7)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     97     97       Iron; alternate (By similarity)     H  
METAL     97     97       Nickel; alternate (By similarity)     H  
METAL     99     99       Iron; alternate (By similarity)     H  
METAL     99     99       Nickel; alternate (By similarity)     H  
METAL     103     103       Iron; alternate (By similarity)     E  
METAL     103     103       Nickel; alternate (By similarity)     E  
METAL     141     141       Iron; alternate (By similarity)     H  
METAL     141     141       Nickel; alternate (By similarity)     H  
SITE (Optional)     96     96       May play a role in metal incorporation in vivo (By similarity)     E  
SITE (Optional)     102     102       May play a role in transmitting local conformational changes (By similarity)     D  
SITE (Optional)     105     105       Important to generate the dianion (By similarity)     R  

Additional information [?]

Size range 165-206 amino acids
Related rules None
Templates Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU): [Recover all]
Fusion None