HAMAP

Accession	MF_01682
Dates	4-NOV-2008 (Created) 18-AUG-2010 (Last updated, Version 10)

Data class

Protein

Predictors

HAMAP; MF_01682; [distribution of match scores in UniProtKB];[seed alignment for MF_01682]

Names

Salvage_MtnD

Identifier

MTND

Protein name

RecName: Full=Acireductone dioxygenase; AltName: Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Short=DHK-MTPene dioxygenase; AltName: Full=Acireductone dioxygenase (Fe(2+)-requiring); Short=ARD'; Short=Fe-ARD; EC=1.13.11.54; AltName: Full=Acireductone dioxygenase (Ni(2+)-requiring); Short=ARD; Short=Ni-ARD; EC=1.13.11.53;

Gene name

mtnD

FUNCTION: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity).

CATALYTIC ACTIVITY: 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 3-(methylthio)propanoate + formate + CO.

CATALYTIC ACTIVITY: 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 4-(methylthio)-2-oxobutanoate + formate.

COFACTOR: Binds 1 iron ion per monomer (By similarity).

COFACTOR: Binds 1 nickel ion per monomer (By similarity).

PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

SUBUNIT: Monomer (By similarity).

SIMILARITY: Belongs to the acireductone dioxygenase (ARD) family.

Pfam	PF03079; ARD; 1;

Amino-acid biosynthesis, Dioxygenase, Iron, Metal-binding, Methionine biosynthesis, Nickel, Oxidoreductase.

GO:0005506; Molecular function: iron ion binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0016151; Molecular function: nickel ion binding.
GO:0019284; Biological process: L-methionine biosynthetic process from S-adenosylmethionine.

From: MTND_KLEOX (Q9ZFE7)
Key		From		To		Description		Condition		FTGroup
METAL		97		97		Iron; alternate (By similarity)		H
METAL		97		97		Nickel; alternate (By similarity)		H
METAL		99		99		Iron; alternate (By similarity)		H
METAL		99		99		Nickel; alternate (By similarity)		H
METAL		103		103		Iron; alternate (By similarity)		E
METAL		103		103		Nickel; alternate (By similarity)		E
METAL		141		141		Iron; alternate (By similarity)		H
METAL		141		141		Nickel; alternate (By similarity)		H
SITE (Optional)		96		96		May play a role in metal incorporation in vivo (By similarity)		E
SITE (Optional)		102		102		May play a role in transmitting local conformational changes (By similarity)		D
SITE (Optional)		105		105		Important to generate the dianion (By similarity)		R

Size range:	165-206 amino acids
Related UniRules:	None
Template:	Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in ERWCT, NOCFA, XANOP
Plasmid encoded:	None