HAMAP

Accession	MF_01684
Dates	19-NOV-2008 (Created) 18-AUG-2010 (Last updated, Version 10)

Data class

Protein

Predictors

HAMAP; MF_01684; [distribution of match scores in UniProtKB];[seed alignment for MF_01684]

Names

Salvage_tnN

Identifier

MTNN

Protein name

RecName: Full=5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase; Short=MTA/SAH nucleosidase; Short=MTAN; EC=3.2.2.9; AltName: Full=5'-methylthioadenosine nucleosidase; Short=MTA nucleosidase; AltName: Full=S-adenosylhomocysteine nucleosidase; Short=AdoHcy nucleosidase; Short=SAH nucleosidase; Short=SRH nucleosidase;

Gene name

mtnN

FUNCTION: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (By similarity).

CATALYTIC ACTIVITY: S-adenosyl-L-homocysteine + H(2)O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.

CATALYTIC ACTIVITY: S-methyl-5'-thioadenosine + H(2)O = S-methyl-5-thio-D-ribose + adenine.

PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Pfam	PF01048; PNP_UDP_1; 1;
TIGRFAMs	TIGR01704; MTA/SAH-Nsdase; 1;

Amino-acid biosynthesis, Hydrolase, Methionine biosynthesis.

GO:0008782; Molecular function: adenosylhomocysteine nucleosidase activity.
GO:0008930; Molecular function: methylthioadenosine nucleosidase activity.
GO:0019284; Biological process: L-methionine biosynthetic process from S-adenosylmethionine.

From: MTNN_ECOLI (P0AF12)
Key		From		To		Description		Condition		FTGroup
REGION		173		174		Substrate binding (By similarity)		M-E
ACT_SITE		12		12		Proton acceptor (By similarity)		E
BINDING (Optional)		78		78		Substrate; via amide nitrogen (By similarity)		G
BINDING (Optional)		152		152		Substrate; via amide nitrogen and carbonyl oxygen (By similarity)		I
BINDING (Optional)		197		197		Substrate (By similarity)		D

Size range:	221-251 amino acids
Related UniRules:	None
Template:	P0AF12 (MTNN_ECOLI); O32028 (MTNN_BACSU); Q9R4A1 (MTNN_KLEPN): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None