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HAMAP rule MF_01687

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General rule information [?]

Accession MF_01687
Dates 19-FEB-2009 (Created)
19-NOV-2022 (Last updated, Version 13)
Name Beta_gal
Scope(s) Bacteria
Template(s) P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA); [ Recover all ]
Triggered by HAMAP; MF_01687 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BGAL
Protein name RecName: Full=Beta-galactosidase;
                 Short=Beta-gal;
                 EC=3.2.1.23;
AltName: Full=Lactase;
Gene name Name=lacZ;

Comments [?]

CATALYTIC ACTIVITY Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per monomer.;
COFACTOR Name=Na(+); Xref=ChEBI:CHEBI:29101; Note=Binds 1 sodium ion per monomer.;
SUBUNITHomotetramer.
SIMILARITYBelongs to the glycosyl hydrolase 2 family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0016798; Molecular function:hydrolase activity, acting on glycosyl bonds

Cross-references [?]

PROSITE PS00719; GLYCOSYL_HYDROL_F2_1; 1;
PROSITE PS00608; GLYCOSYL_HYDROL_F2_2; 1;
Pfam PF02929; Bgal_small_N; 1;
Pfam PF00703; Glyco_hydro_2; 1;
Pfam PF02836; Glyco_hydro_2_C; 1;
Pfam PF02837; Glyco_hydro_2_N; 1;
PRINTS PR00132; GLHYDRLASE2; 1;

Features [?]

From: BGAL_ECOLI (P00722)
Key From To Description Tag Condition FTGroup
BINDING 538 541 /ligand="substrate" E-Y-A-H
ACT_SITE 462 462 /note="Proton donor" E
ACT_SITE 538 538 /note="Nucleophile" E
BINDING 202 202 /ligand="Na(+)"
/ligand_id="ChEBI:CHEBI:29101"
D
BINDING 417 417 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
E
BINDING 419 419 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
H
BINDING 462 462 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
E
BINDING 598 598 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
N
BINDING 602 602 /ligand="Na(+)"
/ligand_id="ChEBI:CHEBI:29101"
F
BINDING 605 605 /ligand="Na(+)"
/ligand_id="ChEBI:CHEBI:29101"
[DN]
BINDING 103 103 /ligand="substrate" N
BINDING 202 202 /ligand="substrate" D
BINDING 462 462 /ligand="substrate" E
BINDING 605 605 /ligand="substrate" [DN]
BINDING 1000 1000 /ligand="substrate" W
SITE 358 358 /note="Transition state stabilizer" H
SITE 392 392 /note="Transition state stabilizer" H

Additional information [?]

Size range 1020-1066 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides.



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