HAMAP

Accession	MF_01688
Dates	8-APR-2009 (Created) 31-OCT-2011 (Last updated, Version 11)

Data class

Protein

Predictors

HAMAP; MF_01688; [distribution of match scores in UniProtKB];[seed alignment for MF_01688]

Names

Biphenyl_BphD

Identifier

BPHD

Protein name

RecName: Full=2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; Short=HOPDA hydrolase; EC=3.7.1.8; AltName: Full=2,6-dioxo-6-phenylhexa-3-enoate hydrolase;

Gene name

bphD

FUNCTION: Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD) (By similarity).

CATALYTIC ACTIVITY: 2,6-dioxo-6-phenylhexa-3-enoate + H(2)O = benzoate + 2-oxopent-4-enoate.

PATHWAY: Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 4/4.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the AB hydrolase superfamily. BphD family.

Pfam	PF00561; Abhydrolase_1; 1;
TIGRFAMs	TIGR03343; Biphenyl_bphD; 1;

Aromatic hydrocarbons catabolism, Hydrolase.

GO:0018771; Molecular function: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity.
GO:0019439; Biological process: aromatic compound catabolic process.

From: BPHD_BURXL (P47229)
Key		From		To		Description		Condition		FTGroup
REGION		42		43		Substrate binding (By similarity)		G-G
ACT_SITE		112		112		Potential		S
ACT_SITE		265		265		Proton acceptor (By similarity)		H
BINDING		51		51		Substrate (By similarity)		N
BINDING		111		111		Substrate (By similarity)		[KN]
BINDING		180		180		Substrate; via carbonyl oxygen (By similarity)		[ST]
BINDING		190		190		Substrate (By similarity)		R
BINDING		266		266		Substrate (By similarity)		W
SITE		112		112		Transition state stabilizer (By similarity)		S

Size range:	286-286 amino acids
Related UniRules:	None
Template:	P47229 (BPHD_BURXL)
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in BURCE, POLNA