HAMAP

Accession	MF_01693
Dates	1-JUL-2009 (Created) 7-OCT-2011 (Last updated, Version 14)

Data class

Protein

Predictors

HAMAP; MF_01693; [distribution of match scores in UniProtKB];[seed alignment for MF_01693]

Names

BioF_aminotrans_2

Identifier

BIOF

Protein name

RecName: Full=8-amino-7-oxononanoate synthase; Short=AONS; EC=2.3.1.47; AltName: Full=7-keto-8-amino-pelargonic acid synthase; Short=7-KAP synthase; AltName: Full=8-amino-7-ketopelargonate synthase; AltName: Full=L-alanine--pimeloyl-CoA ligase;

Gene name

bioF

FUNCTION: Catalyzes the decarboxylative condensation of pimeloyl-CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide (By similarity).

CATALYTIC ACTIVITY: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO(2).

COFACTOR: Pyridoxal phosphate (By similarity).

PATHWAY: Cofactor biosynthesis; biotin biosynthesis; 8-amino-7-oxononanoate from pimeloyl-CoA: step 1/1.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

PROSITE	PS00599; AA_TRANSFER_CLASS_2; 1;
Pfam	PF00155; Aminotran_1_2; 1;
TIGRFAMs	TIGR00858; BioF; 1;

Biotin biosynthesis, Pyridoxal phosphate, Transferase.

GO:0008710; Molecular function: 8-amino-7-oxononanoate synthase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0009102; Biological process: biotin biosynthetic process.

From: BIOF_ECOLI (P12998)
Key		From		To		Description		Condition		FTGroup
REGION		108		109		Pyridoxal phosphate binding (By similarity)		G-[FYW]
REGION		204		207		Pyridoxal phosphate binding (By similarity)		D-[DE]-A-H
REGION (Optional)		233		236		Pyridoxal phosphate binding (By similarity)		T-x-x-K
BINDING		21		21		Substrate (By similarity)		R
BINDING		133		133		Substrate (By similarity)		H
BINDING		179		179		Pyridoxal phosphate (By similarity)		S
BINDING		352		352		Substrate (By similarity)		[TS]
MOD_RES		236		236		N6-(pyridoxal phosphate)lysine (By similarity)		K

Size range:	377-471 amino acids
Related UniRules:	None
Template:	P12998 (BIOF_ECOLI)
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: MF_00668 (bioW); Cter: None
Duplicate:	in POLSJ
Plasmid encoded:	None
Comments:	Biof (8-amino-7-oxononanoate synthase)(TIGR00858) and kbl (2-amino-3-ketobutyrate coenzyme A ligase)(TIGR01822) catalyze very close reactions, and alignment of the amino acid sequences of both enzymes reveals a very close homology, which does not allow to separate them. The only way to create a specific family to the bioF enzymes is to limit the rule to the Proteobacteria. A few Firmicutes (LYSSH and some close orthologs), are annotated as a 8-amino-7-oxononanoate synthase (bioF) due to the characterisation but are not members of the family. The rest of proteins which cannot be differentiates, is annotated as a putative 8-amino-7-oxononanoate synthase ligase in DE line and are not members of the family. Proteins with both activites (TIGR00858/TIGR01825) are annotated as putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase in DE lines and BIKB in ID lines.