HAMAP rule MF_01694
General rule information
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| Accession | MF_01694 |
| Dates | 2-JUL-2009 (Created)
1-JUN-2023 (Last updated, Version 21) |
| Name | BioB |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P12996 (BIOB_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01694 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | BIOB |
| Protein name | RecName: Full=Biotin synthase; EC=2.8.1.6; |
| Gene name | Name=bioB; |
Comments
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| FUNCTION | Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. |
| CATALYTIC ACTIVITY | Reaction=(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe- 2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]- [ferredoxin]; Xref=Rhea:RHEA:22060, Rhea:RHEA-COMP:10000, Rhea:RHEA- COMP:10001, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:17319, ChEBI:CHEBI:29917, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57586, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:149473; EC=2.8.1.6; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| COFACTOR | Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.; |
| PATHWAY | Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 2/2. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the radical SAM superfamily. Biotin synthase family. |
Keywords
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| 2Fe-2S |
| 4Fe-4S |
| Biotin biosynthesis |
| Iron |
| Iron-sulfur |
| Metal-binding |
| S-adenosyl-L-methionine |
| Transferase |
Gene Ontology
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| GO:0004076; Molecular function:biotin synthase activity |
| GO:0005506; Molecular function:iron ion binding |
| GO:0051536; Molecular function:iron-sulfur cluster binding |
| GO:0009102; Biological process:biotin biosynthetic process |
Cross-references
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Features
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| From: BIOB_ECOLI (P12996) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 53 | 53 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 57 | 57 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 60 | 60 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 97 | 97 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
[CS] | ||||||||
| BINDING | 128 | 128 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
[CS] | ||||||||
| BINDING | 188 | 188 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
C | ||||||||
| BINDING | 260 | 260 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" |
[KR] | ||||||||
Additional information
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| Size range | 275-804 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_00834 (bioA) |
| Comments | Bacteroides encode a fusion between BioA (7,8-diamino-pelargonic acid aminotransferase) and BioB. |