HAMAP

Accession	MF_01694
Dates	2-JUL-2009 (Created) 7-OCT-2011 (Last updated, Version 8)

Data class

Protein

Predictors

HAMAP; MF_01694; [distribution of match scores in UniProtKB];[seed alignment for MF_01694]

Names

BioB

Identifier

BIOB

Protein name

RecName: Full=Biotin synthase; EC=2.8.1.6;

Gene name

bioB

FUNCTION: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism (By similarity).

CATALYTIC ACTIVITY: Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine.

COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).

COFACTOR: Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine (By similarity).

PATHWAY: Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the radical SAM superfamily. Biotin synthase family.

Pfam	PF06968; BATS; 1; PF04055; Radical_SAM; 1;
TIGRFAMs	TIGR00433; BioB; 1;

2Fe-2S, 4Fe-4S, Biotin biosynthesis, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Transferase.

GO:0004076; Molecular function: biotin synthase activity.
GO:0005506; Molecular function: iron ion binding.
GO:0051536; Molecular function: iron-sulfur cluster binding.
GO:0009102; Biological process: biotin biosynthetic process.

From: BIOB_ECOLI (P12996)
Key		From		To		Description		Condition		FTGroup
METAL		53		53		Iron-sulfur 1 (4Fe-4S-S-AdoMet) (By similarity)		C
METAL		57		57		Iron-sulfur 1 (4Fe-4S-S-AdoMet) (By similarity)		C
METAL		60		60		Iron-sulfur 1 (4Fe-4S-S-AdoMet) (By similarity)		C
METAL		97		97		Iron-sulfur 2 (2Fe-2S) (By similarity)		[CS]
METAL		128		128		Iron-sulfur 2 (2Fe-2S) (By similarity)		[CS]
METAL		188		188		Iron-sulfur 2 (2Fe-2S) (By similarity)		C
METAL		260		260		Iron-sulfur 2 (2Fe-2S) (By similarity)		[KR]

Size range:	275-804 amino acids
Related UniRules:	None
Template:	P12996 (BIOB_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: MF_00834 (bioA)
Duplicate:	in CORDI, FRASC, PARDP, POLSJ
Plasmid encoded:	in RHILO
Comments:	Bacteroides encode a fusion between BioA (7,8-diamino-pelargonic acid aminotransferase) and BioB.