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HAMAP rule MF_01697

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General rule information [?]

Accession MF_01697
Dates 20-SEP-2010 (Created)
1-JUN-2023 (Last updated, Version 14)
Name MshC
Scope(s) Bacteria
Actinomycetota
Template(s) A0QZY0 (MSHC_MYCS2); P9WJM9 (MSHC_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_01697 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MSHC
Protein name RecName: Full=L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase;
                 Short=L-Cys:GlcN-Ins ligase;
                 EC=6.3.1.13;
AltName: Full=Mycothiol ligase;
                 Short=MSH ligase;
Gene name Name=mshC;

Comments [?]

FUNCTIONCatalyzes the ATP-dependent condensation of GlcN-Ins and L- cysteine to form L-Cys-GlcN-Ins.
CATALYTIC ACTIVITY Reaction=1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside + ATP + L-cysteine = 1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D- glucopyranoside + AMP + diphosphate + H(+); Xref=Rhea:RHEA:26176, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:58886, ChEBI:CHEBI:58887, ChEBI:CHEBI:456215; EC=6.3.1.13;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
SUBUNITMonomer.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.

Keywords [?]


Gene Ontology [?]

GO:0008270; Molecular function:zinc ion binding
GO:0035446; Molecular function:cysteine-glucosaminylinositol ligase activity
GO:0010125; Biological process:mycothiol biosynthetic process

Cross-references [?]

Pfam PF01406; tRNA-synt_1e; 1;
PRINTS PR00983; TRNASYNTHCYS; 1;
NCBIfam TIGR03447; Mycothiol_MshC; 1;

Features [?]

From: MSHC_MYCS2 (A0QZY0)
Key From To Description Tag Condition FTGroup
BINDING 43 46 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
C-G-I-T
BINDING 81 83 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
N-x-T
BINDING 249 251 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
G-x-D
MOTIF 45 55 /note="'HIGH' region" I-T-P-Y-D-[AS]-[AT]-H-[ILM]-G-H
MOTIF 187 192 /note="'ERGGDP' region" [EDQ]-[HR]-G-G-D-P
MOTIF 289 293 /note="'KMSKS' region" K-M-S-K-S
BINDING 43 43 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C
BINDING 231 231 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C
BINDING 256 256 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
H
BINDING 58 58 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
[ST]
BINDING 227 227 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
W
BINDING 283 283 /ligand="L-cysteinyl-5'-AMP"
/ligand_id="ChEBI:CHEBI:144924"
[LIV]

Additional information [?]

Size range 384-520 amino acids
Related rules None
Fusion Nter: None Cter: None



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