HAMAP

Accession	MF_01807
Dates	17-AUG-2003 (Created) 22-JUN-2011 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_01807; [distribution of match scores in UniProtKB];[seed alignment for MF_01807]

Names

Recomb_XerD

Identifier

XERD

Protein name

RecName: Full=Tyrosine recombinase xerD;

Gene name

xerD

FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from xerC binding sites by a short central region, forming the heterotetrameric xerC-xerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. In the complex xerD specifically exchanges the bottom DNA strands (By similarity).

ENZYME REGULATION: FtsK may regulate the catalytic switch between xerC and xerD in the heterotetrameric complex during the two steps of the recombination process (By similarity).

SUBUNIT: Forms a cyclic heterotetrameric complex composed of two molecules of xerC and two molecules of xerD, in which xerC interacts with xerD via its C-terminal region, xerD interacts with xerC via its C-terminal region and so on (By similarity).

FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids (By similarity).

SUBUNIT: Forms a cyclic heterotetrameric complex composed of two molecules of xerC and two molecules of xerD (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the 'phage' integrase family. XerD subfamily.

Pfam	PF00589; Phage_integrase; 1; PF02899; Phage_integr_N; 1;
TIGRFAMs	TIGR02225; Recomb_XerD; 1;

Cytoplasm, DNA recombination, DNA integration, Cell cycle, Cell division, Chromosome partition, DNA-binding.

GO:0009037; Molecular function: tyrosine-based site-specific recombinase activity.
GO:0007059; Biological process: chromosome segregation.
GO:0006310; Biological process: DNA recombination.
GO:0006313; Biological process: transposition, DNA-mediated.
GO:0005737; Cellular component: cytoplasm.

From: XERD_ECOLI (P0A8P8)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		148		148		By similarity		R
ACT_SITE		172		172		By similarity		K
ACT_SITE		244		244		By similarity		H
ACT_SITE		247		247		By similarity		R
ACT_SITE		270		270		By similarity		H
ACT_SITE		279		279		O-(3'-phospho-DNA)-tyrosine intermediate (By similarity)		Y

Size range:	291-335 amino acids
Related UniRules:	MF_01808 (XERC); MF_01816 (XERS); MF_01817 (XERDL)
Template:	P0A8P8 (XERD_ECOLI); P0A2P6 (XERD_SALTY); P46352 (XERD_BACSU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	XerD is highly related to xerC and it is therefore difficult to discriminate them correctly. However, most xerD proteins contain a [ST]RQ tripeptide that allow to distinguish them from xerC proteins.