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HAMAP annotation rule: MF_01818
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General rule information

Accession MF_01818
Dates 12-DEC-2003 (Created)
29-SEP-2011 (Last updated, Version 17)
Data class Protein
Predictors HAMAP; MF_01818; [distribution of match scores in UniProtKB];[seed alignment for MF_01818]
Names RNase_Z/BN


Propagated annotation

Identifier, protein and gene names

case <OC:Enterobacteriaceae>
Identifier RBN
Protein name
RecName: Full=Ribonuclease BN;
Short=RNase BN;
EC=3.1.-.-;
AltName: Full=Ribonuclease Z homolog;
Short=RNase Z homolog;
Gene name rbn

else
Identifier RNZ
Protein name
RecName: Full=Ribonuclease Z;
Short=RNase Z;
EC=3.1.26.11;
AltName: Full=tRNA 3 endonuclease;
AltName: Full=tRNase Z;
Gene name rnz
end case

Comments

case <OC:Enterobacteriaceae>
FUNCTION: Zinc phosphodiesterase, which has both exoribonuclease and endoribonuclease activities (By similarity).

else
FUNCTION: Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (By similarity).
CATALYTIC ACTIVITY: Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.
end case


case <FTGroup:1>
COFACTOR: Binds 2 zinc ions (By similarity).
end case

SUBUNIT: Homodimer (By similarity).

case <OC:Enterobacteriaceae>
SIMILARITY: Belongs to the RNase Z family. RNase BN subfamily.

else
SIMILARITY: Belongs to the RNase Z family.
end case

Cross-references
Pfam PF00753; Lactamase_B; 1;
TIGRFAMs TIGR02651; RNase_Z; 1;
TIGR02649; True_RNase_BN; 1;
Keywords
tRNA processing, Hydrolase, Nuclease, Endonuclease.

case <OC:Enterobacteriaceae>
Exonuclease.
end case


case <FTGroup:1>
Metal-binding, Zinc.
end case

Gene Ontology

case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case


case <OC:Enterobacteriaceae>
GO:0004519; Molecular function: endonuclease activity.
GO:0004527; Molecular function: exonuclease activity.

else
GO:0042781; Molecular function: 3'-tRNA processing endoribonuclease activity.
end case

GO:0008033; Biological process: tRNA processing.
Features
From: RNZ_BACSU (P54548)
Key     From     To       Description   Condition   FTGroup
ACT_SITE     67     67       Proton acceptor (Potential)   D  
 
METAL     63     63       Zinc 1; catalytic (By similarity)   H   1
METAL     65     65       Zinc 1; catalytic (By similarity)   H   1
METAL     67     67       Zinc 2; catalytic (By similarity)   D   1
METAL     68     68       Zinc 2; catalytic (By similarity)   H   1
METAL     140     140       Zinc 1; catalytic (By similarity)   H   1
METAL     211     211       Zinc 1; catalytic (By similarity)   D   1
METAL     211     211       Zinc 2; catalytic (By similarity)   D   1
METAL     269     269       Zinc 2; catalytic (By similarity)   H   1


Additional information

Size range: 220-407 amino acids
Related UniRules: None
Template: P0A8V0 (RBN_ECOLI); Q58897 (RNZ_METJA); P54548 (RNZ_BACSU): [Recover all]
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: <Unknown>
Duplicate: None
Plasmid encoded: None
Comments: Was initially thought to be an arylsulfatase, given its similarity with the atsA family.

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UniProtKB rule member sequences