HAMAP

Accession	MF_01825
Dates	19-MAR-2004 (Created) 2-DEC-2011 (Last updated, Version 13)

Data class

Protein

Predictors

HAMAP; MF_01825; [distribution of match scores in UniProtKB];[seed alignment for MF_01825]

Names

PdxB

Identifier

PDXB

Protein name

RecName: Full=Erythronate-4-phosphate dehydrogenase; EC=1.1.1.290;

Gene name

pdxB

FUNCTION: Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate (By similarity).

CATALYTIC ACTIVITY: 4-phospho-D-erythronate + NAD(+) = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH.

PATHWAY: Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

PROSITE	PS00065; D_2_HYDROXYACID_DH_1; 1; PS00670; D_2_HYDROXYACID_DH_2; 1; PS00671; D_2_HYDROXYACID_DH_3; 1;
Pfam	PF00389; 2-Hacid_dh; 1; PF02826; 2-Hacid_dh_C; 1;
PRINTS	PR00411; PNDRDTASEI; 1;

Cytoplasm, NAD, Oxidoreductase, Pyridoxine biosynthesis.

GO:0016491; Molecular function: oxidoreductase activity.
GO:0008615; Biological process: pyridoxine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PDXB_PSEAE (Q9I3W9)
Key		From		To		Description		Condition		FTGroup
NP_BIND (Optional)		126		127		NAD (By similarity)		Q-V
NP_BIND (Optional)		206		208		NAD (By similarity)		A-S-R
ACT_SITE		208		208		By similarity		R
ACT_SITE		237		237		By similarity		E
ACT_SITE		254		254		Proton donor (By similarity)		H
BINDING (Optional)		45		45		Substrate (By similarity)		[ST]
BINDING (Optional)		66		66		Substrate (By similarity)		T
BINDING (Optional)		146		146		NAD (By similarity)		D
BINDING (Optional)		175		175		NAD; via carbonyl oxygen (By similarity)		T
BINDING (Optional)		232		232		NAD (By similarity)		D
BINDING (Optional)		257		257		NAD; via amide nitrogen (By similarity)		G
BINDING (Optional)		258		258		Substrate (By similarity)		Y

Size range:	345-393 amino acids
Related UniRules:	None
Template:	P05459 (PDXB_ECOLI); Q9I3W9 (PDXB_PSEAE): [Recover all]
Scope:	Bacteria; Bacteroidetes Bacteria; Gammaproteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	The pyridoxal phosphate can be synthetised from two separate branches: the pdxA branch (that requires the gapA (or epd), pdxB, serC and pdxA enzymes) and the pdxJ branch (that requires the dxs and pdxJ enzymes) that start different precursors. Both branches for the last step (pdxH) of the from pyridoxal phosphate pathway.