HAMAP rule MF_01864
General rule information
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Accession | MF_01864 |
Dates | 9-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 20) |
Name | tRNA_metthiotr_MiaB |
Scope(s) |
Bacteria |
Template(s) | P0AEI1 (MIAB_ECOLI); Q9RCI2 (MIAB_SALTY); Q9WZC1 (MIAB_THEMA); O31778 (MIAB_BACSU); [ Recover all ] |
Triggered by |
HAMAP; MF_01864 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | MIAB |
Protein name | RecName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase; EC=2.8.4.3; AltName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase; |
Gene name | Name=miaB; |
Comments
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FUNCTION | Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. |
CATALYTIC ACTIVITY | Reaction=[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)- dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:37067, Rhea:RHEA-COMP:10375, Rhea:RHEA-COMP:10376, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:29917, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:74415, ChEBI:CHEBI:74417; EC=2.8.4.3; |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
SUBUNIT | Monomer. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the methylthiotransferase family. MiaB subfamily. |
Keywords
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Gene Ontology
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GO:0016740; Molecular function:transferase activity |
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
GO:0006400; Biological process:tRNA modification |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS50926; TRAM; 0-1; |
PROSITE | PS01278; MTTASE_RADICAL; 1; |
PROSITE | PS51449; MTTASE_N; 1; |
PROSITE | PS51918; RADICAL_SAM; 1; |
Pfam | PF04055; Radical_SAM; 1; |
Pfam | PF01938; TRAM; 0-1; |
Pfam | PF00919; UPF0004; 1; |
NCBIfam | TIGR01574; MiaB-methiolase; 1; |
NCBIfam | TIGR00089; TIGR00089; 1; |
Features
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From: MIAB_ECOLI (P0AEI1) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 12 | 12 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 49 | 49 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 83 | 83 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 157 | 157 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
BINDING | 161 | 161 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
BINDING | 164 | 164 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 |
Additional information
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Size range | 418-540 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: <Unknown> |