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HAMAP rule MF_01864

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General rule information [?]

Accession MF_01864
Dates 9-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 20)
Name tRNA_metthiotr_MiaB
Scope(s) Bacteria
Template(s) P0AEI1 (MIAB_ECOLI); Q9RCI2 (MIAB_SALTY); Q9WZC1 (MIAB_THEMA); O31778 (MIAB_BACSU); [ Recover all ]
Triggered by HAMAP; MF_01864 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MIAB
Protein name RecName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase;
                 EC=2.8.4.3;
AltName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB;
AltName: Full=tRNA-i(6)A37 methylthiotransferase;
Gene name Name=miaB;

Comments [?]

FUNCTIONCatalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
CATALYTIC ACTIVITY Reaction=[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)- dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:37067, Rhea:RHEA-COMP:10375, Rhea:RHEA-COMP:10376, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:29917, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:74415, ChEBI:CHEBI:74417; EC=2.8.4.3;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the methylthiotransferase family. MiaB subfamily.

Keywords [?]


Gene Ontology [?]

GO:0016740; Molecular function:transferase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0006400; Biological process:tRNA modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS50926; TRAM; 0-1;
PROSITE PS01278; MTTASE_RADICAL; 1;
PROSITE PS51449; MTTASE_N; 1;
PROSITE PS51918; RADICAL_SAM; 1;
Pfam PF04055; Radical_SAM; 1;
Pfam PF01938; TRAM; 0-1;
Pfam PF00919; UPF0004; 1;
NCBIfam TIGR01574; MiaB-methiolase; 1;
NCBIfam TIGR00089; TIGR00089; 1;

Features [?]

From: MIAB_ECOLI (P0AEI1)
Key From To Description Tag Condition FTGroup
BINDING 12 12 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 49 49 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 83 83 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 157 157 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2
BINDING 161 161 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2
BINDING 164 164 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2

Additional information [?]

Size range 418-540 amino acids
Related rules None
Fusion Nter: None Cter: <Unknown>



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