HAMAP

Accession	MF_01864
Dates	9-APR-2009 (Created) 4-JUL-2011 (Last updated, Version 7)

Data class

Protein

Predictors

HAMAP; MF_01864; [distribution of match scores in UniProtKB];[seed alignment for MF_01864]

Names

tRNA_metthiotr_MiaB

Identifier

MIAB

Protein name

RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; EC=2.-.-.-; AltName: Full=tRNA-i(6)A37 methylthiotransferase;

Gene name

miaB

FUNCTION: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine (By similarity).

COFACTOR: Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the methylthiotransferase family. MiaB subfamily.

PROSITE	PS50926; TRAM; 0-1; trigger=PRU00208; PS01278; MTTASE_RADICAL; 1; PS51449; MTTASE_N; 1; trigger=PRU00780;
Pfam	PF04055; Radical_SAM; 1; PF01938; TRAM; 0-1; PF00919; UPF0004; 1;
TIGRFAMs	TIGR01574; MiaB-methiolase; 1; TIGR00089; TIGR00089; 1;

4Fe-4S, Cytoplasm, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Transferase, tRNA processing.

GO:0016740; Molecular function: transferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0006400; Biological process: tRNA modification.
GO:0005737; Cellular component: cytoplasm.

From: MIAB_ECOLI (P0AEI1)
Key		From		To		Description		Condition		FTGroup
METAL		12		12		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		49		49		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		83		83		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		157		157		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		2
METAL		161		161		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		2
METAL		164		164		Iron-sulfur (4Fe-4S-S-AdoMet) (By similarity)		C		2

Size range:	418-540 amino acids
Related UniRules:	None
Template:	P0AEI1 (MIAB_ECOLI); Q9RCI2 (MIAB_SALTY); Q9WZC1 (MIAB_THEMA): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: <Unknown>
Duplicate:	in MYCMM
Plasmid encoded:	in RHOS5, SILPO