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HAMAP rule MF_01865

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General rule information [?]

Accession MF_01865
Dates 15-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 20)
Name MTTase_RimO
Scope(s) Bacteria
Template(s) P0AEI4 (RIMO_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01865 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RIMO
Protein name RecName: Full=Ribosomal protein uS12 methylthiotransferase RimO;
                 Short=uS12 MTTase;
                 Short=uS12 methylthiotransferase;
                 EC=2.8.4.4;
AltName: Full=Ribosomal protein uS12 (aspartate-C(3))-methylthiotransferase;
AltName: Full=Ribosome maturation factor RimO;
Gene name Name=rimO;

Comments [?]

FUNCTIONCatalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12.
CATALYTIC ACTIVITY Reaction=[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L- aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:37087, Rhea:RHEA-COMP:10460, Rhea:RHEA- COMP:10461, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:29917, ChEBI:CHEBI:29961, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:73599; EC=2.8.4.4;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the methylthiotransferase family. RimO subfamily.

Keywords [?]


Gene Ontology [?]

GO:0016740; Molecular function:transferase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0018339; Biological process:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS50926; TRAM; 0-1;
PROSITE PS01278; MTTASE_RADICAL; 1;
PROSITE PS51449; MTTASE_N; 1;
PROSITE PS51918; RADICAL_SAM; 1;
Pfam PF04055; Radical_SAM; 1;
Pfam PF01938; TRAM; 0-1;
Pfam PF00919; UPF0004; 1;
NCBIfam TIGR01125; TIGR01125; 1;
NCBIfam TIGR00089; TIGR00089; 1;

Features [?]

From: RIMO_ECOLI (P0AEI4)
Key From To Description Tag Condition FTGroup
BINDING 17 17 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 53 53 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 82 82 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 150 150 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2
BINDING 154 154 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2
BINDING 157 157 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C 2

Additional information [?]

Size range 401-545 amino acids
Related rules None
Fusion Nter: None Cter: None



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