HAMAP

Accession	MF_01873
Dates	15-OCT-2009 (Created) 18-OCT-2011 (Last updated, Version 7)

Data class

Protein

Predictors

HAMAP; MF_01873; [distribution of match scores in UniProtKB];[seed alignment for MF_01873]

Names

23SrRNA_methyltr_Cfr

Identifier

CFR

Protein name

RecName: Full=Ribosomal RNA large subunit methyltransferase Cfr; EC=2.1.1.224; AltName: Full=23S rRNA (adenine(2503)-C(8))-methyltransferase; AltName: Full=23S rRNA m8A2503 methyltransferase;

Gene name

cfr

FUNCTION: Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics (By similarity).

CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine(2503) in 23S rRNA.

COFACTOR: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (Potential).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the radical SAM superfamily. RlmN family. Cfr subfamily.

Pfam	PF04055; Radical_SAM; 1;
TIGRFAMs	TIGR00048; TIGR00048; 1;
PIRSF	PIRSF006004; CHP00048; 1;

Antibiotic resistance, Cytoplasm, Methyltransferase, rRNA processing, S-adenosyl-L-methionine, Transferase.

4Fe-4S, Iron, Iron-sulfur, Metal-binding.

GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.

GO:0005506; Molecular function: iron ion binding.

GO:0006364; Biological process: rRNA processing.
GO:0005737; Cellular component: cytoplasm.

From: CFR_STASC (Q9FBG4)
Key		From		To		Description		Condition		FTGroup
METAL		112		112		Iron-sulfur (4Fe-4S-S-AdoMet) (Potential)		C		1
METAL		116		116		Iron-sulfur (4Fe-4S-S-AdoMet) (Potential)		C		1
METAL		119		119		Iron-sulfur (4Fe-4S-S-AdoMet) (Potential)		C		1

Size range:	343-350 amino acids
Related UniRules:	MF_01849 (RLMN)
Template:	Q9FBG4 (CFR_STASC)
Scope:	Bacteria; Firmicutes
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in STAAU, STASC, STAWA