HAMAP rule MF_01930
General rule information
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| Accession | MF_01930 |
| Dates | 1-SEP-2014 (Created)
18-JUL-2023 (Last updated, Version 10) |
| Name | PurN |
| Scope(s) |
Bacteria |
| Template(s) | P08179 (PUR3_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01930 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PUR3 |
| Protein name | RecName: Full=Phosphoribosylglycinamide formyltransferase; EC=2.1.2.2; AltName: Full=5'-phosphoribosylglycinamide transformylase; AltName: Full=GAR transformylase; Short=GART; |
| Gene name | Name=purN; |
Comments
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| FUNCTION | Catalyzes the transfer of a formyl group from 10- formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate. |
| CATALYTIC ACTIVITY | Reaction=(6R)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D- ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)- formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide; Xref=Rhea:RHEA:15053, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286, ChEBI:CHEBI:195366; EC=2.1.2.2; |
| PATHWAY | Purine metabolism; IMP biosynthesis via de novo pathway; N(2)- formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D- ribosyl)glycinamide (10-formyl THF route): step 1/1. |
| SIMILARITY | Belongs to the GART family. |
Keywords
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Gene Ontology
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| GO:0004644; Molecular function:phosphoribosylglycinamide formyltransferase activity |
| GO:0006189; Biological process:'de novo' IMP biosynthetic process |
Cross-references
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Features
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| From: PUR3_ECOLI (P08179) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 11 | 13 | /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide" /ligand_id="ChEBI:CHEBI:143788" |
G-[ST]-N | ||||||||
| BINDING | 89 | 92 | /ligand="(6R)-10-formyltetrahydrofolate" /ligand_id="ChEBI:CHEBI:195366" |
[MLF]-[RK]-[IL]-[LVI] | ||||||||
| ACT_SITE | 108 | 108 | /note="Proton donor" | H | ||||||||
| BINDING | 64 | 64 | /ligand="(6R)-10-formyltetrahydrofolate" /ligand_id="ChEBI:CHEBI:195366" |
[RK] | ||||||||
| BINDING | 106 | 106 | /ligand="(6R)-10-formyltetrahydrofolate" /ligand_id="ChEBI:CHEBI:195366" |
N | ||||||||
| SITE | 144 | 144 | /note="Raises pKa of active site His" | D | ||||||||
Additional information
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| Size range | 169-270 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |