HAMAP rule MF_02004
General rule information
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| Accession | MF_02004 |
| Dates | 7-FEB-2006 (Created)
1-JUN-2023 (Last updated, Version 16) |
| Name | Val_tRNA_synth_type1 |
| Scope(s) |
Bacteria |
| Template(s) | P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE); [ Recover all ] |
| Triggered by |
HAMAP; MF_02004 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYV |
| Protein name | RecName: Full=Valine--tRNA ligase; EC=6.1.1.9; AltName: Full=Valyl-tRNA synthetase; Short=ValRS; |
| Gene name | Name=valS; |
Comments
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| FUNCTION | Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA- dependent manner. |
| CATALYTIC ACTIVITY | Reaction=ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl- tRNA(Val); Xref=Rhea:RHEA:10704, Rhea:RHEA-COMP:9672, Rhea:RHEA- COMP:9708, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57762, ChEBI:CHEBI:78442, ChEBI:CHEBI:78537, ChEBI:CHEBI:456215; EC=6.1.1.9; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site. |
| DOMAIN | The C-terminal coiled-coil domain is crucial for aminoacylation activity. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. |
Keywords
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| Cytoplasm |
| Aminoacyl-tRNA synthetase |
| ATP-binding |
| Coiled coil |
| Ligase |
| Nucleotide-binding |
| Protein biosynthesis |
Gene Ontology
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| GO:0004832; Molecular function:valine-tRNA ligase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0006438; Biological process:valyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| Pfam | PF00133; tRNA-synt_1; 1; |
| Pfam | PF08264; Anticodon_1; 1; |
| PRINTS | PR00986; TRNASYNTHVAL; 1; |
| NCBIfam | TIGR00422; ValS; 1; |
| General | Coiled_coil; -; 1-unlimited; |
Features
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| From: SYV_ECOLI (P07118) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 42 | 52 | /note="'HIGH' region" | P-N-[VIL]-T-G-x-[LI]-H-[MLIV]-G-H | ||||||||
| MOTIF | 554 | 558 | /note="'KMSKS' region" | K-[MLF]-[ST]-K-[ST] | ||||||||
| BINDING | 557 | 557 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 822-1020 amino acids |
| Related rules |
MF_02005 |
| Fusion | Nter: None Cter: None |