HAMAP

Accession	MF_02005
Dates	7-FEB-2006 (Created) 21-NOV-2011 (Last updated, Version 9)

Data class

Protein

Predictors

HAMAP; MF_02005; [distribution of match scores in UniProtKB];[seed alignment for MF_02005]

Names

Val_tRNA_synth_type2

Identifier

SYV

Protein name

RecName: Full=Valine--tRNA ligase; EC=6.1.1.9; AltName: Full=Valyl-tRNA synthetase; Short=ValRS;

Gene name

valS

FUNCTION: Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity).

CATALYTIC ACTIVITY: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site (By similarity).

SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.

PROSITE	PS00178; AA_TRNA_LIGASE_I; 1;
Pfam	PF00133; tRNA-synt_1; 1; PF08264; Anticodon_1; 1;
PRINTS	PR00986; TRNASYNTHVAL; 1;
TIGRFAMs	TIGR00422; ValS; 1;

Cytoplasm, Aminoacyl-tRNA synthetase, ATP-binding, Ligase, Nucleotide-binding, Protein biosynthesis.

GO:0004832; Molecular function: valine-tRNA ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006438; Biological process: valyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

From: SYV_RICPR (Q9ZCN6)
Key		From		To		Description		Condition		FTGroup
MOTIF		46		56		"HIGH" region		P-[TYF]-x-[STA]-G-x-x-H-[ILMVP]-G-[HN]
MOTIF		536		540		"KMSKS" region		[KRA]-M-S-K-S
BINDING		539		539		ATP (By similarity)		K

Size range:	751-914 amino acids
Related UniRules:	MF_02004 (SYV)
Template:	P07118 (SYV_ECOLI); P96142 (SYV_THETH); P11931 (SYV_GEOSE): [Recover all]
Scope:	Bacteria; Actinobacteridae Bacteria; Rickettsiales Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None