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HAMAP rule MF_02041

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General rule information [?]

Accession MF_02041
Dates 20-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 7)
Name DusA_subfam
Scope(s) Bacteria
Template(s) Q5SMC7 (DUSAL_THET8); P32695 (DUSA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_02041 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Pseudomonadota>
Identifier DUSA
Protein name RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
                 EC=1.3.1.-;
                 EC=1.3.1.91;
AltName: Full=U20-specific dihydrouridine synthase;
                 Short=U20-specific Dus;
AltName: Full=tRNA-dihydrouridine synthase A;
Gene name Name=dusA;
else
Identifier DUSAL
Protein name RecName: Full=tRNA-dihydrouridine(20/20a) synthase;
                 EC=1.3.1.-;
                 EC=1.3.1.91;
AltName: Full=DusA-like U20-specific dihydrouridine synthase;
                 Short=U20-specific Dus;
end case

Comments [?]

FUNCTIONCatalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA; Xref=Rhea:RHEA:53336, Rhea:RHEA-COMP:13533, Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA; Xref=Rhea:RHEA:53340, Rhea:RHEA-COMP:13533, Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA; Xref=Rhea:RHEA:53344, Rhea:RHEA-COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA; Xref=Rhea:RHEA:53348, Rhea:RHEA-COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210;
SIMILARITYBelongs to the Dus family. DusA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0010181; Molecular function:FMN binding
GO:0000049; Molecular function:tRNA binding
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity
GO:0002943; Biological process:tRNA dihydrouridine synthesis

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
NCBIfam TIGR00742; yjbN; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

From: DUSAL_THET8 (Q5SMC7)
Key From To Description Tag Condition FTGroup
BINDING 10 12 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
P-M-[VML]
BINDING 203 205 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
N-G-G
BINDING 225 226 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
G-R
ACT_SITE 93 93 /note="Proton donor" C
BINDING 63 63 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
Q
BINDING 132 132 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
K
BINDING 164 164 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
H
SITE 90 90 /note="Interacts with tRNA" N
SITE 175 175 /note="Interacts with tRNA; defines subfamily-specific binding signature" K
SITE 178 178 /note="Interacts with tRNA" R
SITE 290 290 /note="Interacts with tRNA; defines subfamily-specific binding signature" R
SITE 293 293 /note="Interacts with tRNA; defines subfamily-specific binding signature" R

Additional information [?]

Size range 288-400 amino acids
Related rules None
Fusion Nter: None Cter: None



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