HAMAP rule MF_02041
General rule information
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Accession | MF_02041 |
Dates | 20-JUL-2015 (Created)
1-JUN-2023 (Last updated, Version 7) |
Name | DusA_subfam |
Scope(s) |
Bacteria |
Template(s) | Q5SMC7 (DUSAL_THET8); P32695 (DUSA_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_02041 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Pseudomonadota> | |
Identifier | DUSA |
Protein name | RecName: Full=tRNA-dihydrouridine(20/20a) synthase; EC=1.3.1.-; EC=1.3.1.91; AltName: Full=U20-specific dihydrouridine synthase; Short=U20-specific Dus; AltName: Full=tRNA-dihydrouridine synthase A; |
Gene name | Name=dusA; |
else | |
Identifier | DUSAL |
Protein name | RecName: Full=tRNA-dihydrouridine(20/20a) synthase; EC=1.3.1.-; EC=1.3.1.91; AltName: Full=DusA-like U20-specific dihydrouridine synthase; Short=U20-specific Dus; |
end case |
Comments
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FUNCTION | Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs. |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH + uridine(20) in tRNA; Xref=Rhea:RHEA:53336, Rhea:RHEA-COMP:13533, Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91; |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH + uridine(20) in tRNA; Xref=Rhea:RHEA:53340, Rhea:RHEA-COMP:13533, Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91; |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH + uridine(20a) in tRNA; Xref=Rhea:RHEA:53344, Rhea:RHEA-COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
CATALYTIC ACTIVITY | Reaction=5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH + uridine(20a) in tRNA; Xref=Rhea:RHEA:53348, Rhea:RHEA-COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; |
COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; |
SIMILARITY | Belongs to the Dus family. DusA subfamily. |
Keywords
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Gene Ontology
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GO:0010181; Molecular function:FMN binding |
GO:0000049; Molecular function:tRNA binding |
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity |
GO:0002943; Biological process:tRNA dihydrouridine synthesis |
Cross-references
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PROSITE | PS01136; UPF0034; 1; |
Pfam | PF01207; Dus; 1; |
NCBIfam | TIGR00742; yjbN; 1; |
PIRSF | PIRSF006621; Dus; 1; |
Features
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From: DUSAL_THET8 (Q5SMC7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 10 | 12 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
P-M-[VML] | ||||||||
BINDING | 203 | 205 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
N-G-G | ||||||||
BINDING | 225 | 226 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
G-R | ||||||||
ACT_SITE | 93 | 93 | /note="Proton donor" | C | ||||||||
BINDING | 63 | 63 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q | ||||||||
BINDING | 132 | 132 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
K | ||||||||
BINDING | 164 | 164 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
H | ||||||||
SITE | 90 | 90 | /note="Interacts with tRNA" | N | ||||||||
SITE | 175 | 175 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | K | ||||||||
SITE | 178 | 178 | /note="Interacts with tRNA" | R | ||||||||
SITE | 290 | 290 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | R | ||||||||
SITE | 293 | 293 | /note="Interacts with tRNA; defines subfamily-specific binding signature" | R |
Additional information
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Size range | 288-400 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |