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HAMAP rule MF_02090

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General rule information [?]

Accession MF_02090
Dates 29-MAR-2017 (Created)
1-JUN-2023 (Last updated, Version 8)
Name NadE_glutamine_dep
Scope(s) Bacteria
Archaea
Template(s) P9WJJ3 (NADE_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_02090 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NADE
Protein name RecName: Full=Glutamine-dependent NAD(+) synthetase;
                 EC=6.3.5.1;
AltName: Full=NAD(+) synthase [glutamine-hydrolyzing];
Gene name Name=nadE;

Comments [?]

FUNCTIONCatalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.
CATALYTIC ACTIVITY Reaction=ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+); Xref=Rhea:RHEA:24384, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58359, ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.5.1;
PATHWAYCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.
SIMILARITYIn the C-terminal section; belongs to the NAD synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0008795; Molecular function:NAD+ synthase activity
GO:0009435; Biological process:NAD biosynthetic process

Cross-references [?]

PROSITE PS50263; CN_HYDROLASE; 1;
Pfam PF00795; CN_hydrolase; 1;
Pfam PF02540; NAD_synthase; 1;
NCBIfam TIGR00552; nadE; 1;
PIRSF PIRSF006630; NADS_GAT; 1;

Features [?]

From: NADE_MYCTU (P9WJJ3)
Key From To Description Tag Condition FTGroup
BINDING 366 373 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[GAP]-x-S-G-G-x-D-S
BINDING 490 493 /ligand="deamido-NAD(+)"
/ligand_id="ChEBI:CHEBI:58437"
/ligand_note="ligand shared between two neighboring subunits"
W-x-T-[YF]
ACT_SITE 52 52 /note="Proton acceptor; for glutaminase activity" E
ACT_SITE 121 121 /note="For glutaminase activity" K
ACT_SITE 176 176 /note="Nucleophile; for glutaminase activity" C
BINDING 127 127 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
Y
BINDING 203 203 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
S
BINDING 209 209 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
[RK]
BINDING 456 456 /ligand="deamido-NAD(+)"
/ligand_id="ChEBI:CHEBI:58437"
/ligand_note="ligand shared between two neighboring subunits"
N
BINDING 480 480 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
T
BINDING 485 485 /ligand="deamido-NAD(+)"
/ligand_id="ChEBI:CHEBI:58437"
/ligand_note="ligand shared between two neighboring subunits"
E
BINDING 635 635 /ligand="deamido-NAD(+)"
/ligand_id="ChEBI:CHEBI:58437"
/ligand_note="ligand shared between two neighboring subunits"
K

Additional information [?]

Size range 510-735 amino acids
Related rules None
Fusion Nter: None Cter: None



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