HAMAP

Accession	MF_02103
Dates	12-AUG-2009 (Created) 22-JUN-2011 (Last updated, Version 5)

Data class

Protein

Predictors

HAMAP; MF_02103; [distribution of match scores in UniProtKB];[seed alignment for MF_02103]

Names

LutB

Identifier

LUTB

Protein name

RecName: Full=Lactate utilization protein B;

Gene name

lutB

FUNCTION: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate (By similarity).

SIMILARITY: Belongs to the lutB/ykgF family.

SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

PROSITE	PS00198; 4FE4S_FER_1; 2; PS51379; 4FE4S_FER_2; 2;
Pfam	PF02589; DUF162; 1;
TIGRFAMs	TIGR00273; TIGR00273; 1;

4Fe-4S, Electron transport, Iron, Iron-sulfur, Metal-binding, Repeat, Transport.

GO:0051536; Molecular function: iron-sulfur cluster binding.
GO:0006089; Biological process: lactate metabolic process.
GO:0019516; Biological process: lactate oxidation.

From: LUTB_BACSU (O07021)
Key		From		To		Description		Condition		FTGroup
DOMAIN		304		334		4Fe-4S ferredoxin-type 1 (By similarity)
DOMAIN		353		382		4Fe-4S ferredoxin-type 2 (By similarity)
METAL		313		313		Iron-sulfur 1 (4Fe-4S) (By similarity)		C
METAL		316		316		Iron-sulfur 1 (4Fe-4S) (By similarity)		C
METAL		319		319		Iron-sulfur 1 (4Fe-4S) (By similarity)		C
METAL		323		323		Iron-sulfur 2 (4Fe-4S) (By similarity)		C
METAL		366		366		Iron-sulfur 2 (4Fe-4S) (By similarity)		C
METAL		369		369		Iron-sulfur 2 (4Fe-4S) (By similarity)		C
METAL		373		373		Iron-sulfur 1 (4Fe-4S) (By similarity)		C

Size range:	468-485 amino acids
Related UniRules:	None
Template:	O07021 (LUTB_BACSU); Q81GA4 (LUTB_BACCR): [Recover all]
Scope:	Bacteria; Firmicutes
Fusion:	Nter: None; Cter: None
Duplicate:	in BACWK
Plasmid encoded:	None
Comments:	The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon (PubMed=19201793). Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli. That's why the profile was not extended to Proteobacteria and this family rule is currently restricted to Firmicutes (in fact seed alignment only contains Bacillales), waiting for characterization in E.coli.