HAMAP rule MF_03004
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_03004 |
| Accession | MF_03004 |
| Dates | 4-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 18) |
| Name | eIF3e |
| Scope(s) |
Eukaryota |
| Template(s) | P60228 (EIF3E_HUMAN); P60229 (EIF3E_MOUSE); O94513 (EIF3E_SCHPO); Q6DRI1 (EI3EA_DANRE); [ Recover all ] |
| Triggered by |
HAMAP; MF_03004 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | EIF3E |
| case <OC:Mammalia> | |
| Protein name | RecName: Full=Eukaryotic translation initiation factor 3 subunit E; Short=eIF3e; AltName: Full=Eukaryotic translation initiation factor 3 subunit 6; AltName: Full=eIF-3 p48; |
| else case <OC:Fungi> | |
| Protein name | RecName: Full=Eukaryotic translation initiation factor 3 subunit E; Short=eIF3e; |
| else | |
| Protein name | RecName: Full=Eukaryotic translation initiation factor 3 subunit E; Short=eIF3e; AltName: Full=Eukaryotic translation initiation factor 3 subunit 6; |
| end case | |
| case <OC:Mammalia> | |
| Gene name | Name=EIF3E; Synonyms=EIF3S6, INT6; |
| else case <OC:Vertebrata> and not <OC:Mammalia> | |
| Gene name | Name=EIF3E; Synonyms=EIF3S6; |
| else case <OC:Caenorhabditis> | |
| Gene name | Name=eif-3.E; |
| else case <OC:Drosophilidae> | |
| Gene name | Name=eIF3e; Synonyms=eIF3-S6, Int6; |
| else case <OC:Fungi> | |
| Gene name | Name=INT6; |
| end case | |
Comments
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| case <OC:Mammalia> | |
| FUNCTION | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| else | |
| FUNCTION | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. |
| end case | |
| case <OC:Mammalia> | |
| SUBUNIT | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2. |
| else case <OC:Vertebrata> and not <OC:Mammalia> | |
| SUBUNIT | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: @gn(EIF3A), @gn(EIF3B), @gn(EIF3C), @gn(EIF3D), @gn(EIF3E), @gn(EIF3F), @gn(EIF3G), @gn(EIF3H), @gn(EIF3I), @gn(EIF3J), @gn(EIF3K), @gn(EIF3L) and @gn(EIF3M). |
| else case <OC:Drosophilidae> | |
| SUBUNIT | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. |
| else | |
| SUBUNIT | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. |
| end case | |
| case <OC:Mammalia> | |
| SUBCELLULAR LOCATION | Cytoplasm. Nucleus. Nucleus, PML body. |
| else case <OC:Vertebrata> and not <OC:Mammalia> | |
| SUBCELLULAR LOCATION | Cytoplasm. Nucleus. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| case <OC:Mammalia> | |
| PTM | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| end case | |
| SIMILARITY | Belongs to the eIF-3 subunit E family. |
Keywords
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| Cytoplasm | |
| Initiation factor | |
| Protein biosynthesis | |
| case <FTTag:acetyl> | |
| Acetylation | |
| end case | |
| case <OC:Vertebrata> | |
| Nucleus | |
| end case | |
| case <OC:Mammalia> | |
| Phosphoprotein | |
| end case | |
Gene Ontology
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| GO:0003743; Molecular function:translation initiation factor activity | |
| GO:0001732; Biological process:formation of cytoplasmic translation initiation complex | |
| GO:0002183; Biological process:cytoplasmic translational initiation | |
| GO:0005737; Cellular component:cytoplasm | |
| case <OCellular component:Vertebrata> | |
| GO:0005634; Cellular component:nucleus | |
| end case | |
| GO:0071540; Cellular component:eukaryotic translation initiation factor 3 complex, eIF3e | |
| GO:0016282; Cellular component:eukaryotic 43S preinitiation complex | |
| GO:0033290; Cellular component:eukaryotic 48S preinitiation complex | |
Cross-references
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Features
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| From: EIF3E_HUMAN (P60228) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Mammalia> | ||||||||||||
| INIT_MET | 1 | 1 | /note="Removed" | M | ||||||||
| REGION | 4 | 128 | /note="Sufficient for interaction with EPAS1" | |||||||||
| REGION | 9 | 195 | /note="Sufficient for interaction with TRIM27" | |||||||||
| REGION | 351 | 445 | /note="Sufficient for interaction with MCM7" | |||||||||
| MOD_RES | 2 | 2 | /note="N-acetylalanine" | acetyl | A | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 357-601 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |