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HAMAP rule MF_03048

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General rule information [?]

Accession MF_03048
Dates 26-FEB-2009 (Created)
20-NOV-2019 (Last updated, Version 7)
Name Urm1
Scope(s) Eukaryota
Template(s) P40554 (URM1_YEAST); Q9BTM9 (URM1_HUMAN); [ Recover all ]
Triggered by HAMAP; MF_03048 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier URM1
case <OC:Fungi> or <OC:Vertebrata>
Protein name RecName: Full=Ubiquitin-related modifier 1;
else
Protein name RecName: Full=Ubiquitin-related modifier 1 homolog;
end case
case <OC:Fungi> or <OC:Vertebrata>
Gene name Name=URM1;
else case <OC:Caenorhabditis>
Gene name Name=urm-1;
else case <OC:Drosophilidae>
Gene name Name=Urm1;
end case

Comments [?]

case <OC:Fungi>
FUNCTIONActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog @gn(UBA4). The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as @gn(AHP1). The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Mammalia>
FUNCTIONActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else case <OC:Vertebrata> or <OC:Viridiplantae>
FUNCTIONActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
else
FUNCTIONActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3/UBA4 homolog. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
end case
PATHWAYtRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
case <OC:Mammalia>
SUBUNITComponent of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
else case <OC:Saccharomyces>
SUBUNITHomodimer; homodimerization may provide an autoprotection to the highly active C-terminal residue before attacking its substrates. Interacts with NCS2 and NCS6. Forms a conjugate with the target protein AHP1.
end case
case <OC:Saccharomyces>
SUBCELLULAR LOCATIONCytoplasm. Nucleus.
else
SUBCELLULAR LOCATIONCytoplasm.
end case
case <OC:Fungi>
PTMC-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of @gn(UBA4), then thiocarboxylated (-COSH) via the rhodanese domain of @gn(UBA4).
else case <OC:Vertebrata>
PTMC-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
else
PTMC-terminal thiocarboxylation occurs in 2 steps, it is first acyl- adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3/UBA4 homolog, then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3/UBA4 homolog.
end case
SIMILARITYBelongs to the URM1 family.

Keywords [?]


Gene Ontology [?]

GO:0005829; Cellular component:cytosol
case <OCellular component:Saccharomyces>
GO:0005634; Cellular component:nucleus
end case
GO:0002098; Biological process:tRNA wobble uridine modification
GO:0032447; Biological process:protein urmylation
GO:0034227; Biological process:tRNA thio-modification

Cross-references [?]

Pfam PF09138; Urm1; 1;

Features [?]

From: URM1_YEAST (P40554)
Key From To Description Tag Condition FTGroup
MOD_RES 99 99 /note="1-thioglycine" G
CROSSLNK 99 99 /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" G

Additional information [?]

Size range 91-142 amino acids
Related rules None
Fusion Nter: None Cter: None



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