HAMAP rule MF_03053
General rule information
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| Accession | MF_03053 |
| Dates | 26-FEB-2009 (Created)
1-JUN-2023 (Last updated, Version 5) |
| Name | CTU1 |
| Scope(s) |
Eukaryota |
| Template(s) | Q7Z7A3 (CTU1_HUMAN); P53088 (CTU1_YEAST); O94282 (CTU1_SCHPO); [ Recover all ] |
| Triggered by |
HAMAP; MF_03053 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CTU1 |
| Protein name | RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1; EC=2.7.7.-; AltName: Full=Cytoplasmic tRNA adenylyltransferase 1; |
| case <OC:Vertebrata> | |
| Protein name | + AltName: Full=ATP-binding domain-containing protein 3; |
| else case <OC:Caenorhabditis> | |
| Protein name | + AltName: Full=Thiolation of uridine in tRNA protein 1; |
| else case <OC:Saccharomyces> | |
| Protein name | + AltName: Full=Thiolation of uridine in cytoplasmic tRNA protein 1; + AltName: Full=Needs CLA4 to survive protein 6; |
| else | |
| Protein name | RecName: Full=Cytoplasmic tRNA 2-thiolation protein 1; EC=2.7.7.-; AltName: Full=Cytoplasmic tRNA adenylyltransferase 1; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=CTU1; Synonyms=ATPBD3, NCS6; |
| else case <OC:Caenorhabditis> | |
| Gene name | Name=tut-1; Synonyms=ctu-1; |
| else case <OC:Saccharomyces> | |
| Gene name | Name=NCS6; Synonyms=CTU1, TUC1; |
| else case <OC:Fungi> or <OC:Viridiplantae> | |
| Gene name | Name=NCS6; Synonyms=CTU1; |
| end case | |
Comments
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| case <OC:Fungi> | |
| FUNCTION | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2- thiolation. May also be involved in protein urmylation. |
| else | |
| FUNCTION | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated @gn(URM1) onto the uridine of tRNAs at wobble position. |
| end case | |
| PATHWAY | tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. |
| case <OC:Mammalia> | |
| SUBUNIT | Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2. |
| else case <OC:Saccharomyces> | |
| SUBUNIT | Interacts with NCS2 and URM1. May act by forming a heterodimer with NCS2. Component of a large molecular weight complex of more than 250 kDa. |
| end case | |
| case <OC:Saccharomyces> | |
| SUBCELLULAR LOCATION | Cytoplasm. Mitochondrion. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| SIMILARITY | Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily. |
Keywords
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| Cytoplasm | |
| case <OC:Saccharomyces> | |
| Mitochondrion | |
| end case | |
| RNA-binding | |
| Transferase | |
| tRNA processing | |
| tRNA-binding | |
Gene Ontology
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| GO:0000049; Molecular function:tRNA binding |
| GO:0016779; Molecular function:nucleotidyltransferase activity |
| GO:0002098; Biological process:tRNA wobble uridine modification |
| GO:0032447; Biological process:protein urmylation |
| GO:0034227; Biological process:tRNA thio-modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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Additional information
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| Size range | 313-443 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |