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HAMAP rule MF_03055

General rule information [?]

Accession	MF_03055
Dates	13-MAR-2009 (Created) 23-MAY-2024 (Last updated, Version 13)
Name	tRNA_methyltr_TrmB_euk
Scope(s)	Eukaryota
Template(s)	Q9UBP6 (TRMB_HUMAN); Q12009 (TRMB_YEAST); [ Recover all ]
Triggered by	HAMAP; MF_03055 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	TRMB
Protein name	RecName: Full=tRNA (guanine-N(7)-)-methyltransferase; EC=2.1.1.33; AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase; AltName: Full=tRNA(m7G46)-methyltransferase;
case <OC:Vertebrata>
Protein name	+ AltName: Full=Methyltransferase-like protein 1;
else case <OC:Fungi>
Protein name	+ AltName: Full=Transfer RNA methyltransferase 8;
end case
case <OC:Vertebrata> or <OC:Dictyostelium>
Gene name	Name=METTL1;
else case <OC:Caenorhabditis>
Gene name	Name=metl-1;
else case <OC:Fungi>
Gene name	Name=TRM8;
end case

Comments [?]

case <OC:Saccharomyces>
FUNCTION	Methyltransferase that catalyzes the formation of N(7)- methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity.
else
FUNCTION	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
end case
CATALYTIC ACTIVITY	Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=2.1.1.33;
PATHWAY	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Vertebrata>
SUBUNIT	Forms a complex with @gn(WDR4).
else case <OC:Fungi>
SUBUNIT	Forms a complex with @gn(TRM82).
end case
SUBCELLULAR LOCATION	Nucleus.
case <FTTag:phospho>
PTM	Phosphorylation at #{Ser-27} inactivates its catalytic activity but does not affect the interaction with @gn(WDR4).
end case
SIMILARITY	Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]

Gene Ontology [?]

GO:0000049; Molecular function:tRNA binding

GO:0008176; Molecular function:tRNA (guanine(46)-N7)-methyltransferase activity

GO:0005634; Cellular component:nucleus

GO:0106004; Biological process:tRNA (guanine-N7)-methylation

Cross-references [?]

Features [?]

From: TRMB_HUMAN (Q9UBP6)

Key

From

Description

Tag

Condition

FTGroup

BINDING

107

108