HAMAP rule MF_03117
General rule information
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| Accession | MF_03117 |
| Dates | 1-APR-2010 (Created)
24-MAY-2024 (Last updated, Version 13) |
| Name | Salvage_MtnC_euk |
| Scope(s) |
Eukaryota |
| Template(s) | Q9UHY7 (ENOPH_HUMAN); P32626 (ENOPH_YEAST); [ Recover all ] |
| Triggered by |
HAMAP; MF_03117 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ENOPH |
| case <OC:Vertebrata> | |
| Protein name | RecName: Full=Enolase-phosphatase E1; EC=3.1.3.77; AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase; AltName: Full=MASA homolog; |
| else case <OC:Saccharomyces> | |
| Protein name | RecName: Full=Enolase-phosphatase E1; EC=3.1.3.77; AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase; AltName: Full=Unknown transcript 4 protein; |
| else | |
| Protein name | RecName: Full=Enolase-phosphatase E1; EC=3.1.3.77; AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase; |
| end case | |
| case <OC:Vertebrata> | |
| Gene name | Name=ENOPH1; Synonyms=MASA; |
| else case <OC:Fungi> | |
| Gene name | Name=UTR4; |
| end case | |
Comments
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| FUNCTION | Bifunctional enzyme that catalyzes the enolization of 2,3- diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK- MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). |
| CATALYTIC ACTIVITY | Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate; Xref=Rhea:RHEA:21700, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:49252, ChEBI:CHEBI:58828; EC=3.1.3.77; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6. |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. Nucleus. |
| SIMILARITY | Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family. |
Keywords
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| Amino-acid biosynthesis | |
| Cytoplasm | |
| Hydrolase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Methionine biosynthesis | |
| Nucleus | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0043715; Molecular function:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity | |
| GO:0043716; Molecular function:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity | |
| GO:0019509; Biological process:L-methionine salvage from methylthioadenosine | |
Cross-references
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| Pfam | PF00702; Hydrolase; 1; |
| PRINTS | PR00413; HADHALOGNASE; 1; |
| NCBIfam | TIGR01691; enolase-ppase; 1; |
| NCBIfam | TIGR01549; HAD-SF-IA-v1; 1; |
Features
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| From: ENOPH_HUMAN (Q9UHY7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 153 | 154 | /ligand="substrate" | S-S | ||||||||
| BINDING | 16 | 16 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 18 | 18 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | 1 | |||||||
| BINDING | 212 | 212 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 187 | 187 | /ligand="substrate" | K | ||||||||
Additional information
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| Size range | 215-1107 amino acids |
| Related rules |
MF_03118 |
| Fusion | Nter: None Cter: None |