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HAMAP rule MF_03123

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General rule information [?]

Accession MF_03123
Dates 23-JUL-2010 (Created)
14-SEP-2023 (Last updated, Version 17)
Name Lipoyl_synth_euk
Scope(s) Eukaryota
Plastid
Template(s) P32875 (LIPA_YEAST); Q8IDQ0 (LIPA_PLAF7); [ Recover all ]
Triggered by
case c? <OC:Eukaryota> or <OG:Plastid>
HAMAP; MF_00206 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Metazoa>
Identifier LIAS
else
Identifier LIPA
end case
case <OG:Organellar chromatophore>
Protein name RecName: Full=Lipoyl synthase, organellar chromatophore;
                 EC=2.8.1.8;
AltName: Full=Lipoate synthase;
                 Short=Lip-syn;
                 Short=LS;
AltName: Full=Lipoic acid synthase;
else case <OC:Apicomplexa>
Protein name RecName: Full=Lipoyl synthase, apicoplast;
                 EC=2.8.1.8;
AltName: Full=Lipoate synthase;
                 Short=Lip-syn;
                 Short=LS;
AltName: Full=Lipoic acid synthase;
else
Protein name RecName: Full=Lipoyl synthase, mitochondrial;
                 EC=2.8.1.8;
AltName: Full=Lipoate synthase;
                 Short=Lip-syn;
                 Short=LS;
AltName: Full=Lipoic acid synthase;
end case
case <OC:Vertebrata>
Gene name Name=LIAS;
else case <OC:Drosophilidae>
Gene name Name=Las;
else case <OC:Apicomplexa>
Gene name Name=lipA;
else case <OC:Saccharomyces>
Gene name Name=LIP5;
end case

Comments [?]

FUNCTIONCatalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
CATALYTIC ACTIVITY Reaction=[[Fe-S] cluster scaffold protein carrying a second [4Fe- 4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'- deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:16585, Rhea:RHEA-COMP:9928, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10475, Rhea:RHEA-COMP:14568, Rhea:RHEA-COMP:14569, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29034, ChEBI:CHEBI:29919, ChEBI:CHEBI:33722, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:78809, ChEBI:CHEBI:83100; EC=2.8.1.8;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
PATHWAYProtein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2.
case <OG:Organellar chromatophore>
SUBCELLULAR LOCATIONPlastid, organellar chromatophore.
else case <OC:Apicomplexa>
SUBCELLULAR LOCATIONPlastid, apicoplast.
else
SUBCELLULAR LOCATIONMitochondrion.
end case
case not <OC:Apicomplexa> and not <OG:Organellar chromatophore> and not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]


Gene Ontology [?]

GO:0016783; Molecular function:sulfurtransferase activity
GO:0016992; Molecular function:lipoate synthase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0009107; Biological process:lipoate biosynthetic process
GO:0009249; Biological process:protein lipoylation
case <OG:Organellar chromatophore>
GO:0070111; Cellular component:organellar chromatophore
else case <OCellular component:Apicomplexa>
GO:0020011; Cellular component:apicoplast
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005739; Cellular component:mitochondrion
end case

Cross-references [?]

Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR00510; lipA; 1;
PIRSF PIRSF005963; Lipoyl_synth; 1;
General Signal; -; 1;
General TransitM; -; 0-1;

Features [?]

From: LIPA_YEAST (P32875)
Key From To Description Tag Condition FTGroup
BINDING 150 150 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 155 155 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 161 161 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 181 181 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C
BINDING 185 185 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C
BINDING 188 188 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"
C
BINDING 396 396 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
S

Additional information [?]

Size range 291-502 amino acids
Related rules MF_03128
MF_03129
Fusion Nter: None Cter: None



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