HAMAP rule MF_03128
General rule information
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| Accession | MF_03128 |
| Dates | 13-AUG-2010 (Created)
3-SEP-2024 (Last updated, Version 18) |
| Name | Lipoyl_synth_plantM |
| Scope(s) |
Eukaryota Viridiplantae |
| Template(s) | Q9ZWT1 (LIAS_ARATH); P32875 (LIPA_YEAST); [ Recover all ] |
| Triggered by |
case c? <OC:Eukaryota>
HAMAP; MF_03128 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LIAS |
| Protein name | RecName: Full=Lipoyl synthase, mitochondrial; EC=2.8.1.8; AltName: Full=Lipoate synthase; Short=Lip-syn; Short=LS; AltName: Full=Lipoic acid synthase; |
| Gene name | Name=LIP1; |
Comments
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| FUNCTION | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. |
| CATALYTIC ACTIVITY | Reaction=[[Fe-S] cluster scaffold protein carrying a second [4Fe- 4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe- 2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:16585, Rhea:RHEA-COMP:9928, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10475, Rhea:RHEA-COMP:14568, Rhea:RHEA-COMP:14569, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29034, ChEBI:CHEBI:29919, ChEBI:CHEBI:33722, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:78809, ChEBI:CHEBI:83100; EC=2.8.1.8; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| PATHWAY | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. |
| SUBCELLULAR LOCATION | Mitochondrion. |
| case not <AnyFeature:TransitM> | |
| MISCELLANEOUS | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
| end case | |
| SIMILARITY | Belongs to the radical SAM superfamily. Lipoyl synthase family. |
Keywords
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Gene Ontology
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| GO:0016783; Molecular function:sulfurtransferase activity |
| GO:0016992; Molecular function:lipoate synthase activity |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
| GO:0009107; Biological process:lipoate biosynthetic process |
| GO:0009249; Biological process:protein lipoylation |
| GO:0005739; Cellular component:mitochondrion |
Cross-references
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| Pfam | PF04055; Radical_SAM; 1; |
| NCBIfam | TIGR00510; LipA; 1; |
| PIRSF | PIRSF005963; Lipoyl_synth; 1; |
| General | TransitM; -; 0-1; |
Features
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| From: LIAS_ARATH (Q9ZWT1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 103 | 103 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 108 | 108 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 114 | 114 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 134 | 134 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 138 | 138 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 141 | 141 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
| BINDING | 350 | 350 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
S | ||||||||
Additional information
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| Size range | 374-399 amino acids |
| Related rules |
MF_03129 MF_03123 |
| Fusion | Nter: None Cter: None |