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HAMAP rule MF_03128

General rule information [?]

Accession	MF_03128
Dates	13-AUG-2010 (Created) 14-SEP-2023 (Last updated, Version 17)
Name	Lipoyl_synth_plantM
Scope(s)	Eukaryota Viridiplantae
Template(s)	Q9ZWT1 (LIAS_ARATH); P32875 (LIPA_YEAST); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_03128 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	LIAS
Protein name	RecName: Full=Lipoyl synthase, mitochondrial; EC=2.8.1.8; AltName: Full=Lipoate synthase; Short=Lip-syn; Short=LS; AltName: Full=Lipoic acid synthase;
Gene name	Name=LIP1;

Comments [?]

FUNCTION	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
CATALYTIC ACTIVITY	Reaction=[[Fe-S] cluster scaffold protein carrying a second [4Fe- 4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'- deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + N(6)-[(R)-dihydrolipoyl]-L-lysyl- [protein] + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:16585, Rhea:RHEA-COMP:9928, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10475, Rhea:RHEA-COMP:14568, Rhea:RHEA-COMP:14569, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:29034, ChEBI:CHEBI:29919, ChEBI:CHEBI:33722, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:78809, ChEBI:CHEBI:83100; EC=2.8.1.8;
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
PATHWAY	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2.
SUBCELLULAR LOCATION	Mitochondrion.
case not <AnyFeature:TransitM>
MISCELLANEOUS	This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITY	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Keywords [?]

S-adenosyl-L-methionine

Gene Ontology [?]

GO:0016783; Molecular function:sulfurtransferase activity

GO:0016992; Molecular function:lipoate synthase activity

GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding

GO:0009107; Biological process:lipoate biosynthetic process

GO:0009249; Biological process:protein lipoylation

GO:0005739; Cellular component:mitochondrion

Cross-references [?]

Pfam	PF04055; Radical_SAM; 1;
NCBIfam	TIGR00510; LipA; 1;
PIRSF	PIRSF005963; Lipoyl_synth; 1;
General	TransitM; -; 0-1;

Features [?]

From: LIAS_ARATH (Q9ZWT1)

Key

From

To

Description

Tag

Condition

FTGroup

BINDING

103

103

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"

C

BINDING

108

108

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"

C

BINDING

114

114

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"

C

BINDING

134

134

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"

C

BINDING

138

138

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"

C

BINDING

141

141

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-S-AdoMet"

C

BINDING

350

350

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"

S

Additional information [?]

Size range	374-399 amino acids
Related rules	MF_03129 MF_03123
Fusion	Nter: None Cter: None

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