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HAMAP rule MF_03133

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General rule information [?]

Accession MF_03133
Dates 22-OCT-2010 (Created)
1-JUN-2023 (Last updated, Version 12)
Name Ala_tRNA_synth_euk
Scope(s) Eukaryota
Plastid
Template(s) P49588 (SYAC_HUMAN); P40825 (SYA_YEAST); P36428 (SYA_ARATH); Q5A8K2 (SYA_CANAL); [ Recover all ]
Triggered by
case c? <OC:Eukaryota> or <OG:Plastid>
HAMAP; MF_00036_B (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Identifier SYAC
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Identifier SYAM
else
Identifier SYA
end case
case <OG:Organellar chromatophore>
Protein name RecName: Full=Alanine--tRNA ligase, organellar chromatophore;
                 EC=6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
                 Short=AlaRS;
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Protein name RecName: Full=Alanine--tRNA ligase, cytoplasmic;
                 EC=6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
                 Short=AlaRS;
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Protein name RecName: Full=Alanine--tRNA ligase, mitochondrial;
                 EC=6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
                 Short=AlaRS;
else
Protein name RecName: Full=Alanine--tRNA ligase;
                 EC=6.1.1.7;
AltName: Full=Alanyl-tRNA synthetase;
                 Short=AlaRS;
end case
case <OC:Vertebrata>
Gene name Name=AARS;
else case <OC:Drosophilidae> and <AnyFeature:TransitM>
Gene name Name=AlaRS-m;
else case <OC:Drosophilidae> and not <AnyFeature:TransitM>
Gene name Name=AlaRS;
else case <OC:Caenorhabditis>
Gene name Name=aars;
else case <OC:Fungi>
Gene name Name=ALA1;
end case

Comments [?]

FUNCTIONCatalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
CATALYTIC ACTIVITY Reaction=ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl- tRNA(Ala); Xref=Rhea:RHEA:12540, Rhea:RHEA-COMP:9657, Rhea:RHEA- COMP:9923, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57972, ChEBI:CHEBI:78442, ChEBI:CHEBI:78497, ChEBI:CHEBI:456215; EC=6.1.1.7;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
case <OC:Vertebrata>
SUBUNITMonomer. Interacts with ANKRD16; the interaction is direct.
else
SUBUNITMonomer.
end case
case <OG:Organellar chromatophore>
SUBCELLULAR LOCATIONPlastid, organellar chromatophore.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
SUBCELLULAR LOCATIONCytoplasm.
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
SUBCELLULAR LOCATIONMitochondrion.
else
SUBCELLULAR LOCATIONMitochondrion. Cytoplasm.
end case
DOMAINConsists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
case <OC:Mammalia>
PTMISGylated.
end case
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis
RNA-binding
tRNA-binding
case <FTGroup:1>
Metal-binding
Zinc
end case
case <OG:Organellar chromatophore>
Organellar chromatophore
Plastid
else case (<OC:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
Cytoplasm
else case (<OC:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
Mitochondrion
else
Cytoplasm
Mitochondrion
end case
case <FTTag:acetyl>
Acetylation
end case
case <OC:Mammalia>
Ubl conjugation
end case

Gene Ontology [?]

GO:0004813; Molecular function:alanine-tRNA ligase activity
GO:0005524; Molecular function:ATP binding
GO:0008270; Molecular function:zinc ion binding
case <OG:Organellar chromatophore>
GO:0006419; Biological process:alanyl-tRNA aminoacylation
GO:0070111; Cellular component:organellar chromatophore
else case (<OCellular component:Metazoa> or <OC:Amoebozoa>) and not <AnyFeature:TransitM>
GO:0006419; Biological process:alanyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm
else case (<OCellular component:Metazoa> or <OC:Amoebozoa>) and <AnyFeature:TransitM>
GO:0070143; Biological process:mitochondrial alanyl-tRNA aminoacylation
GO:0005739; Cellular component:mitochondrion
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0006419; Biological process:alanyl-tRNA aminoacylation
GO:0070143; Biological process:mitochondrial alanyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm
GO:0005739; Cellular component:mitochondrion
end case

Cross-references [?]

Pfam PF02272; DHHA1; 1;
Pfam PF01411; tRNA-synt_2c; 1;
Pfam PF07973; tRNA_SAD; 1;
PRINTS PR00980; TRNASYNTHALA; 1;
NCBIfam TIGR00344; AlaS; 1;
PROSITE PS50860; AA_TRNA_LIGASE_II_ALA; 1;
General TransitM; -; 0-1;

Features [?]

From: SYAC_HUMAN (P49588)
Key From To Description Tag Condition FTGroup
BINDING 605 605 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
BINDING 609 609 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
BINDING 723 723 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 1
BINDING 727 727 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
case <OC:Mammalia>
MOD_RES 1 1 /note="N-acetylmethionine" acetyl M
end case

Additional information [?]

Size range 433-1408 amino acids
Related rules MF_03134
Fusion Nter: None Cter: None



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