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HAMAP rule MF_03143

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General rule information [?]

Accession MF_03143
Dates 16-FEB-2011 (Created)
1-JUN-2023 (Last updated, Version 15)
Name Pentafunct_AroM
Scope(s) Eukaryota
Fungi
Template(s) P07547 (ARO1_EMENI); P08566 (ARO1_YEAST); [ Recover all ]
Triggered by HAMAP; MF_03143 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ARO1
Protein name RecName: Full=Pentafunctional AROM polypeptide;
                 Includes:
RecName: Full=3-dehydroquinate synthase;
                 Short=DHQS;
                 EC=4.2.3.4;
                 Includes:
RecName: Full=3-phosphoshikimate 1-carboxyvinyltransferase;
                 EC=2.5.1.19;
AltName: Full=5-enolpyruvylshikimate-3-phosphate synthase;
                 Short=EPSP synthase;
                 Short=EPSPS;
                 Includes:
RecName: Full=Shikimate kinase;
                 Short=SK;
                 EC=2.7.1.71;
                 Includes:
RecName: Full=3-dehydroquinate dehydratase;
                 Short=3-dehydroquinase;
                 EC=4.2.1.10;
                 Includes:
RecName: Full=Shikimate dehydrogenase;
                 EC=1.1.1.25;
case <OC:Trichocomaceae>
Gene name Name=aroM;
else case <OC:Saccharomycetales>
Gene name Name=ARO1;
else case <OC:Schizosaccharomyces>
Gene name Name=aro1;
else case <OC:Neurospora>
Gene name Name=aro-1;
end case

Comments [?]

FUNCTIONThe AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.
CATALYTIC ACTIVITY Reaction=7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3- dehydroquinate + phosphate; Xref=Rhea:RHEA:21968, ChEBI:CHEBI:32364, ChEBI:CHEBI:43474, ChEBI:CHEBI:58394; EC=4.2.3.4;
CATALYTIC ACTIVITY Reaction=3-dehydroquinate = 3-dehydroshikimate + H2O; Xref=Rhea:RHEA:21096, ChEBI:CHEBI:15377, ChEBI:CHEBI:16630, ChEBI:CHEBI:32364; EC=4.2.1.10;
CATALYTIC ACTIVITY Reaction=NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH; Xref=Rhea:RHEA:17737, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630, ChEBI:CHEBI:36208, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.25;
CATALYTIC ACTIVITY Reaction=ATP + shikimate = 3-phosphoshikimate + ADP + H(+); Xref=Rhea:RHEA:13121, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:36208, ChEBI:CHEBI:145989, ChEBI:CHEBI:456216; EC=2.7.1.71;
CATALYTIC ACTIVITY Reaction=3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate; Xref=Rhea:RHEA:21256, ChEBI:CHEBI:43474, ChEBI:CHEBI:57701, ChEBI:CHEBI:58702, ChEBI:CHEBI:145989; EC=2.5.1.19;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.;
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYIn the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.
SIMILARITYIn the 2nd section; belongs to the EPSP synthase family.
SIMILARITYIn the 3rd section; belongs to the shikimate kinase family.
SIMILARITYIn the 4th section; belongs to the type-I 3-dehydroquinase family.
SIMILARITYIn the C-terminal section; belongs to the shikimate dehydrogenase family.

Keywords [?]

Amino-acid biosynthesis
Aromatic amino acid biosynthesis
ATP-binding
Cytoplasm
Kinase
Lyase
Metal-binding
Multifunctional enzyme
NADP
Nucleotide-binding
Oxidoreductase
Transferase
Zinc

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0003855; Molecular function:3-dehydroquinate dehydratase activity
GO:0003856; Molecular function:3-dehydroquinate synthase activity
GO:0003866; Molecular function:3-phosphoshikimate 1-carboxyvinyltransferase activity
GO:0005524; Molecular function:ATP binding
GO:0046872; Molecular function:metal ion binding
GO:0004764; Molecular function:shikimate 3-dehydrogenase (NADP+) activity
GO:0004765; Molecular function:shikimate kinase activity
GO:0009073; Biological process:aromatic amino acid family biosynthetic process

Cross-references [?]

PROSITE PS01028; DEHYDROQUINASE_I; 1;
PROSITE PS00104; EPSP_SYNTHASE_1; 1;
PROSITE PS00885; EPSP_SYNTHASE_2; 1;
PROSITE PS01128; SHIKIMATE_KINASE; 1;
Pfam PF01487; DHquinase_I; 1;
Pfam PF00275; EPSP_synthase; 1;
Pfam PF01488; Shikimate_DH; 1;
Pfam PF08501; Shikimate_dh_N; 1;
Pfam PF01202; SKI; 1;
Pfam PF01761; DHQ_synthase; 1;
PRINTS PR01100; SHIKIMTKNASE; 1;
NCBIfam TIGR01356; aroA; 1;
NCBIfam TIGR01357; aroB; 1;
NCBIfam TIGR01093; aroD; 1;
NCBIfam TIGR01809; Shik-DH-AROM; 1;
PIRSF PIRSF001455; DHQ_synth; 1;
PIRSF PIRSF000514; Pentafunct_AroM; 1;

Features [?]

From: ARO1_EMENI (P07547)
Key From To Description Tag Condition FTGroup
BINDING 44 46 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 D-[TSDQ]-[NRT]
BINDING 81 84 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 E-x(2)-K
BINDING 114 116 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 G-G-[VI]
BINDING 139 140 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 T-[TS]
BINDING 179 182 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 [FYW]-[LI]-x-[TS]
BINDING 870 877 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 G-M-R-[GA]-[AST]-G-K-[ST]
REGION Nter 384 /note="3-dehydroquinate synthase"
BINDING 194 197 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 E-[VIL]-[IV]-K
BINDING 264 268 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 R-[NT]-[LI]-[LV]-N
REGION 397 842 /note="EPSP synthase"
REGION 863 1055 /note="Shikimate kinase"
REGION 1056 1276 /note="3-dehydroquinase"
REGION 1289 Cter /note="Shikimate dehydrogenase"
ACT_SITE 260 260 /note="Proton acceptor; for 3-dehydroquinate synthase activity" E
ACT_SITE 275 275 /note="Proton acceptor; for 3-dehydroquinate synthase activity" H
ACT_SITE 824 824 /note="For EPSP synthase activity" C
ACT_SITE 1179 1179 /note="Proton acceptor; for 3-dehydroquinate dehydratase activity" H
ACT_SITE 1207 1207 /note="Schiff-base intermediate with substrate; for 3- dehydroquinate dehydratase activity" [KR]
BINDING 194 194 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 E
BINDING 271 271 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H
BINDING 287 287 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H
BINDING 119 119 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 D
BINDING 130 130 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 R
BINDING 146 146 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 D
BINDING 152 152 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 K
BINDING 161 161 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 K
BINDING 162 162 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 N
BINDING 190 190 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 N
BINDING 250 250 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 K
BINDING 271 271 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 H
BINDING 287 287 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 H
BINDING 356 356 /ligand="7-phospho-2-dehydro-3-deoxy-D-arabino- heptonate"
/ligand_id="ChEBI:CHEBI:58394"		 K

Additional information [?]

Size range 1523-1715 amino acids
Related rules MF_00109
MF_00110
MF_00210
MF_00214
MF_00222
Fusion Nter: None Cter: None



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