HAMAP rule MF_03148

General rule information [?]

Accession	MF_03148
Dates	9-AUG-2011 (Created) 3-SEP-2024 (Last updated, Version 15)
Name	HAM1_NTPase
Scope(s)	Eukaryota
Template(s)	Q9BY32 (ITPA_HUMAN); Q9D892 (ITPA_MOUSE); P47119 (ITPA_YEAST); [ Recover all ]
Triggered by	HAMAP; MF_03148 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	ITPA
Protein name	RecName: Full=Inosine triphosphate pyrophosphatase; Short=ITPase; Short=Inosine triphosphatase; EC=3.6.1.9; AltName: Full=Non-canonical purine NTP pyrophosphatase; AltName: Full=Non-standard purine NTP pyrophosphatase; AltName: Full=Nucleoside-triphosphate diphosphatase; AltName: Full=Nucleoside-triphosphate pyrophosphatase; Short=NTPase;
case <OC:Vertebrata>
Gene name	Name=ITPA;
else case <OC:Caenorhabditis>
Gene name	Name=hap-1;
else case <OC:Saccharomycotina>
Gene name	Name=HAM1;
end case

Comments [?]

case <OC:Vertebrata>
FUNCTION	Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else case <OC:Saccharomyces>
FUNCTION	Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
else
FUNCTION	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
end case
CATALYTIC ACTIVITY	Reaction=a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- phosphate + diphosphate + H(+); Xref=Rhea:RHEA:23996, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58043, ChEBI:CHEBI:61557; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:23997;
CATALYTIC ACTIVITY	Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'- deoxyribonucleoside 5'-phosphate + diphosphate + H(+); Xref=Rhea:RHEA:44644, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:65317; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44645;
CATALYTIC ACTIVITY	Reaction=ITP + H2O = IMP + diphosphate + H(+); Xref=Rhea:RHEA:29399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58053, ChEBI:CHEBI:61402; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29400;
CATALYTIC ACTIVITY	Reaction=dITP + H2O = dIMP + diphosphate + H(+); Xref=Rhea:RHEA:28342, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61194, ChEBI:CHEBI:61382; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28343;
CATALYTIC ACTIVITY	Reaction=XTP + H2O = XMP + diphosphate + H(+); Xref=Rhea:RHEA:28610, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:61314; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28611;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).;
SUBUNIT	Homodimer.
case <OC:Fungi>
SUBCELLULAR LOCATION	Cytoplasm. Nucleus.
else
SUBCELLULAR LOCATION	Cytoplasm.
end case
SIMILARITY	Belongs to the HAM1 NTPase family.

Keywords [?]

case <FTGroup:1>
Acetylation
end case
case <OC:Fungi>
Nucleus
end case
Cytoplasm
Hydrolase
Magnesium
Manganese
Metal-binding
Nucleotide metabolism
Nucleotide-binding

Gene Ontology [?]

case <OCellular component:Fungi>
GO:0005634; Cellular component:nucleus
end case
GO:0005737; Cellular component:cytoplasm
GO:0047429; Molecular function:nucleoside triphosphate diphosphatase activity
GO:0009204; Biological process:deoxyribonucleoside triphosphate catabolic process

Cross-references [?]

Pfam	PF01725; Ham1p_like; 1;
NCBIfam	TIGR00042; TIGR00042; 1;

Features [?]

From: ITPA_HUMAN (Q9BY32)

Key

From

Description

Tag

Condition

FTGroup

case <OC:Mammalia>

INIT_MET

Nter

/note="Removed"

MOD_RES

Nter+1

/note="N-acetylalanine"

end case

BINDING

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

T-x-N-x-x-K

BINDING

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

D-[TS]

BINDING

149

152

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

F-G-W-[DEN]

BINDING

177

178

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

H-R

BINDING

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

BINDING

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

BINDING

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

BINDING

172

/ligand="ITP"
/ligand_id="ChEBI:CHEBI:61402"

[KR]

Additional information [?]

Size range	183-234 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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