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HAMAP rule MF_03148
General rule information
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Accession | MF_03148 |
Dates | 9-AUG-2011 (Created)
1-JUN-2023 (Last updated, Version 14) |
Name | HAM1_NTPase |
Scope(s) |
Eukaryota |
Template(s) | Q9BY32 (ITPA_HUMAN); Q9D892 (ITPA_MOUSE); P47119 (ITPA_YEAST); [ Recover all ] |
Triggered by |
HAMAP; MF_03148 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ITPA |
Protein name | RecName: Full=Inosine triphosphate pyrophosphatase; Short=ITPase; Short=Inosine triphosphatase; EC=3.6.1.9; AltName: Full=Non-canonical purine NTP pyrophosphatase; AltName: Full=Non-standard purine NTP pyrophosphatase; AltName: Full=Nucleoside-triphosphate diphosphatase; AltName: Full=Nucleoside-triphosphate pyrophosphatase; Short=NTPase; |
case <OC:Vertebrata> | |
Gene name | Name=ITPA; |
else case <OC:Caenorhabditis> | |
Gene name | Name=hap-1; |
else case <OC:Saccharomycotina> | |
Gene name | Name=HAM1; |
end case |
Comments
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case <OC:Vertebrata> | |
FUNCTION | Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
else case <OC:Saccharomyces> | |
FUNCTION | Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
else | |
FUNCTION | Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
end case | |
CATALYTIC ACTIVITY | Reaction=a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- phosphate + diphosphate + H(+); Xref=Rhea:RHEA:23996, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58043, ChEBI:CHEBI:61557; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:23997; |
CATALYTIC ACTIVITY | Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'- deoxyribonucleoside 5'-phosphate + diphosphate + H(+); Xref=Rhea:RHEA:44644, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:65317; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44645; |
CATALYTIC ACTIVITY | Reaction=H2O + ITP = diphosphate + H(+) + IMP; Xref=Rhea:RHEA:29399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58053, ChEBI:CHEBI:61402; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29400; |
CATALYTIC ACTIVITY | Reaction=dITP + H2O = dIMP + diphosphate + H(+); Xref=Rhea:RHEA:28342, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61194, ChEBI:CHEBI:61382; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28343; |
CATALYTIC ACTIVITY | Reaction=H2O + XTP = diphosphate + H(+) + XMP; Xref=Rhea:RHEA:28610, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:61314; EC=3.6.1.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28611; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).; |
SUBUNIT | Homodimer. |
case <OC:Fungi> | |
SUBCELLULAR LOCATION | Cytoplasm. Nucleus. |
else | |
SUBCELLULAR LOCATION | Cytoplasm. |
end case | |
SIMILARITY | Belongs to the HAM1 NTPase family. |
Keywords
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case <FTGroup:1> | |
Acetylation | |
end case | |
case <OC:Fungi> | |
Nucleus | |
end case | |
Cytoplasm | |
Hydrolase | |
Magnesium | |
Manganese | |
Metal-binding | |
Nucleotide metabolism | |
Nucleotide-binding |
Gene Ontology
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case <OCellular component:Fungi> | |
GO:0005634; Cellular component:nucleus | |
end case | |
GO:0005737; Cellular component:cytoplasm | |
GO:0047429; Molecular function:nucleoside triphosphate diphosphatase activity | |
GO:0009204; Biological process:deoxyribonucleoside triphosphate catabolic process |
Cross-references
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Features
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From: ITPA_HUMAN (Q9BY32) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case <OC:Mammalia> | ||||||||||||
INIT_MET | Nter | Nter | /note="Removed" | M | 1 | |||||||
MOD_RES | Nter+1 | Nter+1 | /note="N-acetylalanine" | A | 1 | |||||||
end case | ||||||||||||
BINDING | 14 | 19 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
T-x-N-x-x-K | ||||||||
BINDING | 72 | 73 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
D-[TS] | ||||||||
BINDING | 149 | 152 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
F-G-W-[DEN] | ||||||||
BINDING | 177 | 178 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
H-R | ||||||||
BINDING | 44 | 44 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
BINDING | 72 | 72 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
BINDING | 56 | 56 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
K | ||||||||
BINDING | 172 | 172 | /ligand="ITP" /ligand_id="ChEBI:CHEBI:61402" |
[KR] |
Additional information
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Size range | 183-234 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |