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HAMAP rule MF_03152

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General rule information [?]

Accession MF_03152
Dates 17-OCT-2011 (Created)
13-OCT-2023 (Last updated, Version 12)
Name TRM5
Scope(s) Eukaryota
Template(s) P38793 (TRM5_YEAST); Q32P41 (TRM5_HUMAN); [ Recover all ]
Triggered by HAMAP; MF_03152 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRM5
case <OC:Saccharomyces>
Protein name RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
                 EC=2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5;
                 Flags: Precursor;
else case <OC:Fungi>
Protein name RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
                 EC=2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5;
else
Protein name RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
                 EC=2.1.1.228;
AltName: Full=M1G-methyltransferase;
AltName: Full=tRNA [GM37] methyltransferase;
AltName: Full=tRNA methyltransferase 5 homolog;
end case
case <OC:Vertebrata>
Gene name Name=TRMT5; Synonyms=TRM5;
else case <OC:Fungi>
Gene name Name=TRM5;
end case

Comments [?]

case <OC:Saccharomyces>
FUNCTIONSpecifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.
else
FUNCTIONSpecifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
end case
CATALYTIC ACTIVITY Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)- methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228;
SUBUNITMonomer.
SUBCELLULAR LOCATIONMitochondrion matrix. Nucleus. Cytoplasm. Note=Predominantly in the mitochondria and in the nucleus.
SIMILARITYBelongs to the TRM5 / TYW2 family.

Keywords [?]

Cytoplasm
Methyltransferase
Mitochondrion
Nucleus
S-adenosyl-L-methionine
Transferase
tRNA processing
case <OC:Saccharomyces>
Transit peptide
end case

Gene Ontology [?]

GO:0005759; Cellular component:mitochondrial matrix
GO:0005634; Cellular component:nucleus
GO:0005737; Cellular component:cytoplasm
GO:0030488; Biological process:tRNA methylation
GO:0052906; Molecular function:tRNA (guanine(37)-N1)-methyltransferase activity

Cross-references [?]

PROSITE PS51684; SAM_MT_TRM5_TYW2; 1;
Pfam PF02475; Met_10; 1;
General TransitM; -; 0-1;

Features [?]

From: TRM5_YEAST (P38793)
Key From To Description Tag Condition FTGroup
case <OC:Saccharomyces>
TRANSIT Nter 44 /note="Mitochondrion"
CHAIN 45 Cter /note=""
end case
BINDING 307 308 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [DE]-[ILM]
BINDING 335 336 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [DE]-[AGNSV]
BINDING 268 268 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [HKR]
BINDING 399 399 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 N

Additional information [?]

Size range 360-781 amino acids
Related rules None
Fusion Nter: None Cter: None



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