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HAMAP rule MF_03176
General rule information
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| Accession | MF_03176 |
| Dates | 31-JUL-2013 (Created)
19-NOV-2022 (Last updated, Version 10) |
| Name | PIF1 |
| Scope(s) |
Eukaryota |
| Template(s) | P07271 (PIF1_YEAST); Q9H611 (PIF1_HUMAN); Q9UUA2 (PIF1_SCHPO); [ Recover all ] |
| Triggered by |
HAMAP; MF_03176 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PIF1 |
| RecName: Full=ATP-dependent DNA helicase PIF1;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.6.4.12">3.6.4.12</a>;<br /> AltName: Full=DNA repair and recombination helicase PIF1; | |
| Gene name | Name=PIF1; |
Comments
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| case <OC:Saccharomycetales> | |
| FUNCTION | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing. |
| else case <OC:Mammalia> | |
| FUNCTION | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. |
| else | |
| FUNCTION | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| case <OC:Saccharomycetales> or <OC:Mammalia> | |
| SUBUNIT | Monomer. Interacts with telomerase. |
| else | |
| SUBUNIT | Monomer. |
| end case | |
| SUBCELLULAR LOCATION | Nucleus. Mitochondrion. |
| SIMILARITY | Belongs to the helicase family. PIF1 subfamily. |
Keywords
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| ATP-binding | |
| DNA damage | |
| DNA recombination | |
| DNA repair | |
| DNA-binding | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Hydrolase | |
| Mitochondrion | |
| Nucleotide-binding | |
| Nucleus | |
Gene Ontology
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| case <OCellular component:Saccharomycetales> or <OC:Mammalia> | |
| GO:0051880; Molecular function:G-quadruplex DNA binding | |
| GO:0010521; Molecular function:telomerase inhibitor activity | |
| GO:0032211; Biological process:negative regulation of telomere maintenance via telomerase | |
| end case | |
| GO:0005739; Cellular component:mitochondrion | |
| GO:0005634; Cellular component:nucleus | |
| GO:0005524; Molecular function:ATP binding | |
| GO:0043139; Molecular function:5'-3' DNA helicase activity | |
| GO:0006310; Biological process:DNA recombination | |
| GO:0006281; Biological process:DNA repair | |
| GO:0000002; Biological process:mitochondrial genome maintenance | |
Cross-references
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| Pfam | PF05970; PIF1; 1-2; |
Features
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| From: PIF1_YEAST (P07271) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 258 | 265 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x-A-G-T-G-K-S | ||||||||
| DNA_BIND | 727 | 746 | [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F | |||||||||
Additional information
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| Size range | 593-1113 amino acids |
| Related rules |
MF_03177 |
| Fusion | Nter: None Cter: None |