HAMAP rule MF_03211
General rule information
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| Accession | MF_03211 |
| Dates | 11-NOV-2015 (Created)
19-NOV-2022 (Last updated, Version 10) |
| Name | RNA_acetyltr_Nat10 |
| Scope(s) |
Eukaryota |
| Template(s) | P53914 (NAT10_YEAST); P87115 (NAT10_SCHPO); Q9H0A0 (NAT10_HUMAN); [ Recover all ] |
| Triggered by |
HAMAP; MF_03211 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NAT10 |
| Protein name | RecName: Full=RNA cytidine acetyltransferase; EC=2.3.1.-; AltName: Full=18S rRNA cytosine acetyltransferase; |
| case <OC:Mammalia> | |
| Protein name | + AltName: Full=N-acetyltransferase 10; |
| end case | |
| case <OC:Vertebrata> or <OC:Fungi> | |
| Gene name | Name=NAT10; |
| else case <OC:Caenorhabditis> | |
| Gene name | Name=nath-10; |
| end case | |
Comments
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| case <OC:Vertebrata> | |
| FUNCTION | RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA- binding adapter protein @gn(THUMPD1) for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. |
| else case <OC:Fungi> | |
| FUNCTION | RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA- binding adapter protein @gn(TAN1) for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. |
| else | |
| FUNCTION | RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding adapter protein for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = ADP + an N(4)-acetylcytidine in 18S rRNA + CoA + H(+) + phosphate; Xref=Rhea:RHEA:51424, Rhea:RHEA-COMP:13575, Rhea:RHEA-COMP:13576, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216; |
| CATALYTIC ACTIVITY | Reaction=a cytidine in tRNA + acetyl-CoA + ATP + H2O = ADP + an N(4)- acetylcytidine in tRNA + CoA + H(+) + phosphate; Xref=Rhea:RHEA:53876, Rhea:RHEA-COMP:13670, Rhea:RHEA-COMP:13671, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216; |
| case <OC:Vertebrata> | |
| SUBUNIT | Interacts with @gn(THUMPD1). |
| else case <OC:Fungi> | |
| SUBUNIT | Interacts with @gn(TAN1). |
| end case | |
| SUBCELLULAR LOCATION | Nucleus, nucleolus. |
| SIMILARITY | Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily. |
Keywords
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Gene Ontology
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| GO:0005730; Cellular component:nucleolus |
| GO:0005524; Molecular function:ATP binding |
| GO:0008080; Molecular function:N-acetyltransferase activity |
| GO:0051391; Biological process:tRNA acetylation |
| GO:0000154; Biological process:rRNA modification |
| GO:0042274; Biological process:ribosomal small subunit biogenesis |
Cross-references
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| PROSITE | PS51186; GNAT; 1; |
| Pfam | PF08351; DUF1726; 1; |
| Pfam | PF13718; GNAT_acetyltr_2; 1; |
| Pfam | PF05127; Helicase_RecD; 1; |
| Pfam | PF13725; tRNA_bind_2; 1; |
Features
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| From: NAT10_YEAST (P53914) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 286 | 295 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-R-G-K-S-A-[TAS]-[IVLM]-G-[IVLM] | ||||||||
| BINDING | 638 | 640 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
[IV]-A-[ATV] | ||||||||
| BINDING | 645 | 651 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
x(2)-[MLA]-[GN]-Y-G-x | ||||||||
| BINDING | 475 | 475 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
| BINDING | 739 | 739 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
[RKN] | ||||||||
Additional information
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| Size range | 825-1233 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |