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HAMAP rule MF_03216

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General rule information [?]

Accession MF_03216
Dates 7-JUL-2016 (Created)
19-NOV-2022 (Last updated, Version 8)
Name PLMT
Scope(s) Eukaryota
Template(s) Q9UBM1 (PEMT_HUMAN); P05375 (PLMT_YEAST); Q7S5W9 (PLMT_NEUCR); Q61907 (PEMT_MOUSE); [ Recover all ]
Triggered by HAMAP; MF_03216 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Fungi> or <OC:Viridiplantae>
Identifier PLMT
Protein name RecName: Full=Phosphatidyl-N-methylethanolamine N-methyltransferase;
                 EC=2.1.1.71;
AltName: Full=Phospholipid methyltransferase;
                 Short=PLMT;
else
Identifier PEMT
Protein name RecName: Full=Phosphatidylethanolamine N-methyltransferase;
                 Short=PEAMT;
                 Short=PEMT;
                 EC=2.1.1.17;
                 EC=2.1.1.71;
AltName: Full=Phospholipid methyltransferase;
                 Short=PLMT;
end case
case <OC:Vertebrata>
Gene name Name=PEMT;
end case

Comments [?]

case <OC:Fungi> or <OC:Viridiplantae>
FUNCTIONCatalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC).
else
FUNCTIONCatalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3- phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2- diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2- diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L- homocysteine in each step.
end case
CATALYTIC ACTIVITY Reaction=1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S- adenosyl-L-methionine = 1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32735, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64572, ChEBI:CHEBI:64573; EC=2.1.1.71;
CATALYTIC ACTIVITY Reaction=1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S- adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32739, ChEBI:CHEBI:15378, ChEBI:CHEBI:57643, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64572;
case not (<OC:Fungi> or <OC:Viridiplantae>)
CATALYTIC ACTIVITY Reaction=a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L- methionine = 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:11164, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64573, ChEBI:CHEBI:64612; EC=2.1.1.17;
end case
PATHWAYPhospholipid metabolism; phosphatidylcholine biosynthesis.
case <OC:Vertebrata>
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Note=Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
else case <OC:Viridiplantae>
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Multi-pass membrane protein.
else
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.
end case
SIMILARITYBelongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.

Keywords [?]

Endoplasmic reticulum
Lipid biosynthesis
Lipid metabolism
Membrane
Methyltransferase
case not <OC:Viridiplantae>
Mitochondrion
end case
Phospholipid biosynthesis
Phospholipid metabolism
S-adenosyl-L-methionine
Transferase
Transmembrane
Transmembrane helix

Gene Ontology [?]

GO:0005789; Cellular component:endoplasmic reticulum membrane
case not <OCellular component:Viridiplantae>
GO:0031966; Cellular component:mitochondrial membrane
end case
GO:0080101; Molecular function:phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
GO:0000773; Molecular function:phosphatidyl-N-methylethanolamine N-methyltransferase activity
case not <OCellular component:Fungi>
GO:0004608; Molecular function:phosphatidylethanolamine N-methyltransferase activity
end case
GO:0006656; Biological process:phosphatidylcholine biosynthetic process

Cross-references [?]

PROSITE PS51599; SAM_PEMT_PEM2; 1;
PROSITE PS50244; S5A_REDUCTASE; 1;
Pfam PF04191; PEMT; 1;
PIRSF PIRSF005444; PEMT; 1;

Features [?]

From: PEMT_HUMAN (Q9UBM1)
Key From To Description Tag Condition FTGroup
TOPO_DOM Nter 12 /note="Lumenal"
INTRAMEM 13 33 /note="Helical"
TOPO_DOM 34 45 /note="Lumenal"
TRANSMEM 46 66 /note="Helical"
TOPO_DOM 67 93 /note="Cytoplasmic"
TRANSMEM 94 114 /note="Helical"
TOPO_DOM 115 157 /note="Lumenal"
TRANSMEM 158 178 /note="Helical"
TOPO_DOM 179 Cter /note="Cytoplasmic"
BINDING 98 100 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 x(2)-G
BINDING 180 181 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 E-x

Additional information [?]

Size range 164-248 amino acids
Related rules None
Fusion Nter: None Cter: None



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