HAMAP rule MF_04063
General rule information
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| Accession | MF_04063 |
| Dates | 30-MAR-2017 (Created)
19-NOV-2022 (Last updated, Version 8) |
| Name | INFV_PA |
| Scope(s) |
Viruses Orthomyxoviridae |
| Template(s) | P03433 (PA_I34A1); [ Recover all ] |
| Triggered by |
HAMAP; MF_04063 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PA |
| Protein name | RecName: Full=Polymerase acidic protein; EC=3.1.-.-; AltName: Full=RNA-directed RNA polymerase subunit P2; |
| Gene name | Name=PA; |
Comments
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| FUNCTION | Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. |
| COFACTOR | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 manganese ions per subunit |
| SUBUNIT | Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus). |
| SUBCELLULAR LOCATION | Host cytoplasm. Host nucleus. Note=PB1 and PA are transported in the host nucleus as a complex. |
| PTM | Phosphorylated on serines and threonines by host kinases, including human casein kinase II. |
| SIMILARITY | Belongs to the influenza viruses PA family. |
Keywords
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Gene Ontology
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| GO:0030430; Cellular component:host cell cytoplasm |
| GO:0042025; Cellular component:host cell nucleus |
| GO:0003723; Molecular function:RNA binding |
| GO:0075526; Biological process:cap snatching |
| GO:0039523; Biological process:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity |
| GO:0006351; Biological process:DNA-templated transcription |
Cross-references
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| Pfam | PF00603; Flu_PA; 1; |
Features
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| From: PA_I34A1 (P03433) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 124 | 139 | /note="Nuclear localization signal 1 (NLS1)" | |||||||||
| MOTIF | 184 | 247 | /note="Nuclear localization signal 2 (NLS2)" | |||||||||
| BINDING | 41 | 41 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
H | ||||||||
| BINDING | 80 | 80 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
E | ||||||||
| BINDING | 108 | 108 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
D | ||||||||
| BINDING | 108 | 108 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="2" |
D | ||||||||
| BINDING | 119 | 119 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
E | ||||||||
| BINDING | 120 | 120 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" /ligand_label="1" |
[IV] | ||||||||
Additional information
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| Size range | 709-726 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |