HAMAP proteomes
Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4))

General information

Species:  Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4))
Species code: AGRVS
Taxonomy: Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium (TaxID: 311402) [NEWT/ NCBI]
Description: Agrobacterium are Gram-negative, motile, soil-dwelling plant pathogens with the species name given based on the disease phenotype associated with the bacteria. They invade the crown, roots and stems of a great variety of plants via wounds causing tumors. The diseases are crown gall, hairy root, and cane gall. Some strains possess a wide host range, whereas other possess a very limited host range. The tumor is correlated with the presence of a large tumor-inducing plasmid (Ti plasmid) in the bacteria. Thus A.tumefaciens causes crown gall on many dicotyledonous plants; A.rubi causes crown gall on raspberries; A.vitis gall formation on grapes; A.rhizogenes causes hairy roots; A.radiobacter is avirulent. However the ability to cause disease is associated with transmissible plasmids, and this grouping is easily disrupted when plasmids move from one strain to another. More recently Agrobacterium have been classified into 3 biovars based on physiological and biochemical phenotypes without consideration of disease; the 2 classification systems are not compatible. There is now a fully sequenced representative of each biovar publicly available.

Crown galls are formed when Agrobacterium with a a tumor-inducing Ti plasmid infects a wounded plant. A portion of the Ti-plasmid DNA (T-DNA) is transferred from the bacterial plasmid into the host plant cell where the T-DNA is integrated into host plant chromosomal DNA by an illegitimate recombination process. Some of the transferred genes encode enzymes responsible for the synthesis of plant hormones that cannot be controlled by the plant, causing abnormal cell divisions that produce a plant gall. Other transferred genes encode biosynthetic enzymes capable of transforming plant metabolites into a class of compounds called opines that are not catabolizable by the host plant, but are catabolizable by the tumor-inducing bacterial strain. A.vitis strain S4 is a virulent biovar III strain isolated from the Vitis vinifera (grape) cv. Izsaki Sarfeher crown gall in Kecskemet/Orgavony, Hungary in 1981. It produces the opine vitopine. A.vitis strains not only cause galls on grapevines but also necrosis on grapevine roots and a hypersensitive response on nonhost plants (adapted from PubMed 11524123 and 19251847).
Properties: Presence of flagella: Yes
Human pathogen: No
Interaction: Plant pathogen in Vitis (grape)
Number of membranes: 2

Genome(s) sequenced

Strain:    S4 / ATCC BAA-846
Genome structure:
• Chromosome 1 EMBL accession number CP000633 (circular; 3,726,375 bp) (download entry)
• Chromosome 2 EMBL accession number CP000634 (circular; 1,283,187 bp) (download entry)
• Plasmid pAtS4b EMBL accession number CP000635 (circular; 130,435 bp) (download entry)
• Plasmid pAtS4c EMBL accession number CP000636 (circular; 211,620 bp) (download entry)
• Plasmid pTiS4 EMBL accession number CP000637 (circular; 258,824 bp) (download entry)
• Plasmid pAtS4e EMBL accession number CP000638 (circular; 631,775 bp) (download entry)
• Plasmid pAtS4a EMBL accession number CP000639 (circular; 78,730 bp) (download entry)
[1] Pubmed=19251847;
Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A., Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W. ;
"Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria.";
J. Bacteriol. 191:2501-2511(2009).
Web links:
Official genome site(s):
Other web sites:
Entrez Genome Project: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genomeprj&cmd=ShowDetailView&TermToSearch=13372