HAMAP rule MF_00001
General rule information
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| Accession | MF_00001 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 39) |
| Name | Asp_carb_tr |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A786 (PYRB_ECOLI); P05654 (PYRB_BACSU); Q58976 (PYRB_METJA); Q55338 (PYRB_SULAC); [ Recover all ] |
| Triggered by |
HAMAP; MF_00001 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PYRB |
| Protein name | RecName: Full=Aspartate carbamoyltransferase; EC=2.1.3.2; AltName: Full=Aspartate transcarbamylase; Short=ATCase; |
| Gene name | Name=pyrB; |
Comments
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| FUNCTION | Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway. |
| CATALYTIC ACTIVITY | Reaction=carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+); Xref=Rhea:RHEA:20013, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:32814, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228; EC=2.1.3.2; |
| PATHWAY | Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. |
| case <OC:Bacteria> | |
| SUBUNIT | Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2). |
| else case <OC:Archaea> | |
| SUBUNIT | Heterooligomer of catalytic and regulatory chains. |
| end case | |
| SIMILARITY | Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family. |
Keywords
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Gene Ontology
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| GO:0004070; Molecular function:aspartate carbamoyltransferase activity |
| GO:0044205; Biological process:'de novo' UMP biosynthetic process |
Cross-references
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| Pfam | PF00185; OTCace; 1; |
| NCBIfam | TIGR00670; asp_carb_tr; 1; |
| PROSITE | PS00097; CARBAMOYLTRANSFERASE; 1; |
Features
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| From: PYRB_ECOLI (P0A786) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 55 | 55 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
R | ||||||||
| BINDING | 56 | 56 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
T | ||||||||
| BINDING | 85 | 85 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
K | ||||||||
| BINDING | 106 | 106 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
R | ||||||||
| BINDING | 135 | 135 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
H | ||||||||
| BINDING | 138 | 138 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
Q | ||||||||
| BINDING | 168 | 168 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
R | ||||||||
| BINDING | 230 | 230 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
R | ||||||||
| BINDING | 268 | 268 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
[GASL] | ||||||||
| BINDING | 269 | 269 | /ligand="carbamoyl phosphate" /ligand_id="ChEBI:CHEBI:58228" |
P | ||||||||
Additional information
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| Size range | 291-475 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_00002 (pyrI) |